OER - BIOL 2420 Laboratory Manual

by Sherry Smith

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Publisher
North Central Texas College
Subjects
Biology
Licence
CC BY 4.0, as LibreTexts states it
Who can read
Tweens profiles and older, judged from its title and subjects

Contents

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  1. Front Matter
  2. Non-Science Majors Microbiology Laboratory Manual
  3. InfoPage
  4. Table of Contents
  5. Licensing
  6. Preface
  7. THECB ACGM Course Laboratory Learning Outcome to Exercise Correlation Tables
  8. Acknowledgements
  9. 1: Section One - Getting Started In The Microbiology Laboratory
  10. 1.1: Exercise #1 - Laboratory Safety
  11. 1.1.1: Microbiology Safety Guidelines
  12. 1.1.2: Laboratory Safety Equipment
  13. 1.1.3: Exercise #1 Lab Wrap-Up
  14. 1.2: Exercise #2 - Microscopy
  15. 1.2.1: Transporting the Microscope
  16. 1.2.2: Identifying the Components of the Compound Microscope
  17. 1.2.3: Calculating Total Magnification
  18. 1.2.4: Using the Compound Microscope
  19. 1.2.4.1: Observing the "Letter e"
  20. 1.2.4.2: Observing a "whitefish blastodisc"
  21. 1.2.4.3: Observing a "human blood smear"
  22. 1.2.4.4: Observing "Balantidium coli"
  23. 1.2.4.5: Observing a "bacterial smear"
  24. 1.2.5: Exercise #2 Lab Wrap-Up
  25. 1.3: Exercise #3 - Growth Media and Inoculating Tools
  26. 1.3.1: Types of Media - Chemical Composition
  27. 1.3.2: Types of Media - Physical Characteristics
  28. 1.3.3: Types of Media – Functional/Application Classifications
  29. 1.3.4: Inoculating Tools
  30. 1.3.5: Exercise #3 Lab Wrap-Up
  31. 1.4: Exercise #4 - Aseptic Techniques and Aseptic Transfer of Bacteria
  32. 1.4.1: Aseptic Technique and Subculturing Bacteria
  33. 1.4.2: Becoming Familiar with Aseptic Techniques
  34. 1.4.3: Aseptically Transferring Bacteria From Broth Culture to Sterile Broth
  35. 1.4.4: Aseptically Transferring Bacteria From Broth Culture to Sterile Agar Slant
  36. 1.4.5: Aseptically Transferring Bacteria From Broth Culture to Sterile Agar Plate
  37. 1.4.6: Exercise #4 Lab Wrap-Up
  38. 1.5: Exercise #5 - Isolation Techniques
  39. 1.5.1: Streak Plate
  40. 1.5.2: Performing the Streak Plate
  41. 1.5.3: Exercise #5 Lab Wrap-Up
  42. 1.6: Section One Review
  43. 1.6.1: Matching
  44. 1.6.2: Fill-In-The-Blank
  45. 1.6.3: True/False
  46. 1.6.4: Multiple Choice / Multiple Select
  47. 2: Section Two - Visualizing Bacteria (Staining Bacteria)
  48. 2.1: Exercise #6 - Bacterial Cellular Morphology and Structure
  49. 2.1.1: Cellular Morphology and Structure
  50. 2.1.2: Bacterial Cellular Arrangement
  51. 2.1.3: Exercise #6 Lab Wrap-Up
  52. 2.2: Exercise #7 - Specimen Preparation and Simple Staining
  53. 2.2.1: Specimen Preparation and Simple Staining
  54. 2.2.2: Cheek Smear Activity
  55. 2.2.3: Bacterial Smear Activity
  56. 2.2.4: Exercise #7 Lab Wrap-Up
  57. 2.3: Exercise #8 - Differential Staining- Gram Staining
  58. 2.3.1: Gram Stain
  59. 2.3.2: Gram Stain Activity
  60. 2.3.3: Smear Visualization
  61. 2.3.4: Exercise #8 Lab Wrap-Up
  62. 2.4: Exercise #9 - Differential Staining- Acid-Fast Stain
  63. 2.4.1: Acid-Fast Stain
  64. 2.4.2: Acid-Fast Stain Activity
  65. 2.4.3: Smear Visualization
  66. 2.4.4: Exercise #9 Lab Wrap-Up
  67. 2.5: Exercise #10 - Structural Stain- Endospore Stain
  68. 2.5.1: Endospore Stain
  69. 2.5.2: Endospore Stain Activity
  70. 2.5.3: Smear Visualization
  71. 2.5.4: Exercise #10 Lab Wrap-Up
  72. 2.6: Exercise #11 - Structural Stain- Capsule Stain
  73. 2.6.1: Capsule Stain
  74. 2.6.2: Capsule Stain Activity
  75. 2.6.3: Smear Visualization
  76. 2.6.4: Exercise #11 - Lab Wrap-Up
  77. 2.7: Section Two Review
  78. 2.7.1: Matching
  79. 2.7.2: Fill-In-The-Bank
  80. 2.7.3: True/False
  81. 2.7.4: Multiple Choice / Multiple Select
  82. 3: Section Three Assessing Metabolic Characteristics of Bacteria (Biochemical Tests, Selective and Differential Media)
  83. 3.1: Exercise #12 - Carbohydrate Fermentation Tests
  84. 3.1.1: Carbohydrate Fermentation Test
  85. 3.1.2: Carbohydrate Fermentation Test Activity
  86. 3.1.3: Exercise #12 - Lab Wrap-Up
  87. 3.2: Exercise #13 - Triple Sugar Iron Agar Test
  88. 3.2.1: Triple Sugar Iron Agar Test
  89. 3.2.2: Triple Sugar Iron Agar Test Activity
  90. 3.2.3: Exercise #13 - Lab Wrap-Up
  91. 3.3: Exercise #14 - SIM Medium Test
  92. 3.3.1: SIM Medium Test
  93. 3.3.2: SIM Medium Test Activity
  94. 3.3.3: Exercise #14 - Lab Wrap-Up
  95. 3.4: Exercise 15 - IMViC Tests
  96. 3.4.1: Indole Test
  97. 3.4.2: Indole Test Activity
  98. 3.4.3: MRVP
  99. 3.4.4: Methyl Red (MR) and Voges-Proskauer (VP) Test Activity
  100. 3.4.5: Citrate Test
  101. 3.4.6: Citrate Test Activity
  102. 3.4.7: Exercise #15 - Lab Wrap-Up
  103. 3.5: Exercise #16 - Respiration Tests
  104. 3.5.1: Oxidase Test
  105. 3.5.2: Oxidase Test Activity
  106. 3.5.3: Catalase Test
  107. 3.5.4: Catalase Test Activity
  108. 3.5.5: Nitrate Reduction Test
  109. 3.5.6: Nitrate Reduction Test Activity
  110. 3.5.7: Exercise #16 - Lab Wrap-Up
  111. 3.6: Exercise #17 - Urease Test
  112. 3.6.1: Urease Test
  113. 3.6.2: Urease Test Activity
  114. 3.6.3: Exercise #17 - Lab Wrap-Up
  115. 3.7: Exercise #18 - Coagulase Test
  116. 3.7.1: Coagulase Test
  117. 3.7.2: Coagulase Test Activity
  118. 3.7.3: Exercise #18 - Lab Wrap-Up
  119. 3.8: Exercise #19 - Casease Test
  120. 3.8.1: Casease Test
  121. 3.8.2: Casease Test Activity
  122. 3.8.3: Exercise #19 - Lab Wrap-Up
  123. 3.9: Exercise #20 - Starch Hydrolysis Test
  124. 3.9.1: Starch Hydrolysis Test
  125. 3.9.2: Starch Hydrolysis Test Activity
  126. 3.9.3: Exercise #20 - Lab Wrap-Up
  127. 3.10: Exercise #21 - Blood Agar
  128. 3.10.1: Blood Agar Test
  129. 3.10.2: Blood Agar Test Activity
  130. 3.10.3: Exercise #21 - Lab Wrap-Up
  131. 3.11: Exercise #22 MacConkey Agar
  132. 3.11.1: MacConkey Agar
  133. 3.11.2: MacConkey Agar Activity
  134. 3.11.3: Exercise #22 - Lab Wrap-Up
  135. 3.12: Exercise #23 - Mannitol Salt Agar
  136. 3.12.1: Mannitol Salt Agar Test
  137. 3.12.2: Mannitol Salt Agar Test Activity
  138. 3.12.3: Exercise #23 - Lab Wrap-Up
  139. 3.13: Section Three Review
  140. 3.13.1: Matching
  141. 3.13.2: Fill-In-The-Blank
  142. 3.13.3: True/False
  143. 3.13.4: Multiple Choice / Multiple Select
  144. 4: Section Four - Enumerating Bacteria
  145. 4.1: Exercise #24 - Viable Plate Counts
  146. 4.1.1: Serial Dilutions and Viable Plate Counts
  147. 4.1.2: Serial Dilutions and Viable Plate Counts Activity
  148. 4.1.3: Exercise #24 - Lab Wrap-Up
  149. 4.2: Exercise #25- Direct Microscopic Count
  150. 4.2.1: Direct Microscopic Count
  151. 4.2.2: Direct Microscopic Count Activity
  152. 4.2.3: Exercise #25 - Lab Wrap-Up
  153. 4.3: Section Four Review
  154. 4.3.1: Matching
  155. 4.3.2: Fill-In-The-Blank
  156. 4.3.3: True/False
  157. 4.3.4: Multiple Choice / Multiple Select
  158. 5: Section Five - Factors Affecting Microbial Growth
  159. 5.1: Exercise #26 - Effect of Temperature on Microbial Growth
  160. 5.1.1: Temperature and Microbial Growth
  161. 5.1.2: Assessing Effects of Temperature on Microbial Growth Activity
  162. 5.1.3: Assessing Effects of Temperature on Phenotypic Expression Activity
  163. 5.1.4: Exercise #26 - Lab Wrap-Up
  164. 5.2: Exercise #27 - Effect of pH on Microbial Growth
  165. 5.2.1: pH and Microbial Growth
  166. 5.2.2: Assessing Effects of pH on Microbial Growth Activity
  167. 5.2.3: Exercise #27 - Lab Wrap-Up
  168. 5.3: Exercise #28 - Effect of Antiseptics and Disinfectants on Microbial Growth
  169. 5.3.1: Antiseptics and Disinfectants
  170. 5.3.2: Assessing the Effects of Antiseptics and Disinfectants on Microbial Growth Activity
  171. 5.3.3: Exercise #28 - Lab Wrap-Up
  172. 5.4: Exercise #29 - Antimicrobial Sensitivity Test (Kirby-Bauer Method)
  173. 5.4.1: Antimicrobial Susceptibility Test
  174. 5.4.2: Antimicrobial Sensitivity Test (Kirby-Bauer Method) Activity
  175. 5.4.3: Exercise #29 - Lab Wrap-Up
  176. 5.5: Section Five Review
  177. 5.5.1: Matching
  178. 5.5.2: Fill-In-The-Blank
  179. 5.5.3: True/False
  180. 5.5.4: Multiple Choice / Multiple Select
  181. 6: Section Six - Eukaryotic Pathogens (A Survey of Fungal, Protozoan, and Helminth Pathogens)
  182. 6.1: Exercise #30 - Fungal Pathogens
  183. 6.1.1: Fungi
  184. 6.1.2: Survey of Clinically Significant Yeasts
  185. 6.1.3: Survey of Clinically Significant Molds
  186. 6.1.4: Exercise #30 - Lab Wrap-Up
  187. 6.2: Exercise #31 - Protozoan Parasites
  188. 6.2.1: Protozoans
  189. 6.2.2: Subphylum Sarcodina (Amoeboids)
  190. 6.2.3: Subphylum Mastigophora (Flagellates)
  191. 6.2.4: Phylum Ciliophora (Ciliates)
  192. 6.2.5: Phylum Apicomplexa (Sporozoa)
  193. 6.2.6: Exercise #31 - Lab Wrap-Up
  194. 6.3: Exercise #32 - Helminth Pathogens
  195. 6.3.1: Helminths
  196. 6.3.2: Trematodes (Flukes)
  197. 6.3.3: Survey of Trematodes
  198. 6.3.4: Cestodes (Tapeworms)
  199. 6.3.5: Survey of Cestodes
  200. 6.3.6: Nematodes (Roundworms)
  201. 6.3.7: Survey of Nematodes
  202. 6.3.8: Exercise #32 - Lab Wrap-Up
  203. 6.4: Section Six Review
  204. 6.4.1: Matching
  205. 6.4.2: Fill-In-The-Blank
  206. 6.4.3: True-False
  207. 6.4.4: Multiple Choice / Multiple Select
  208. 6.4.5: Identifying Parasites
  209. Back Matter
  210. Index
  211. Glossary
  212. Detailed Licensing
  213. Detailed Licensing