5.2: Exercise #27 - Effect of pH on Microbial Growth

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    THECB - ACGM Lab Learning Outcome #5 Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

    Laboratory Exercise Learning Outcomes
    1. Define the following groups used to characterize bacteria based on their pH preferences; acidophile, neutrophile, alkaliphile.
    2. Describe how microorganisms are affected by pH.
    3. Perform an experiment to determine the pH preferences for a given set of bacterial species and appropriately classify the bacteria based on those preferences.

    Materials Needed:

    • Bunsen burner
    • Inoculating loop
    • Broth bacterial cultures (Lactobacillus acidophilis, Staphylococcus aureus, Alcaligenes faecalis)
    • 3 sterile nutrient broth cultures pH 3
    • 3 sterile nutrient broth cultures pH 5
    • 3 sterile nutrient broth cultures pH 7
    • 3 sterile nutrient broth cultures pH 9
    • 3 sterile nutrient broth cultures pH 11

    This page titled 5.2: Exercise #27 - Effect of pH on Microbial Growth was last modified on Sat, 25 Jul 2026 03:42:56 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.