3.10: Exercise #21 - Blood Agar

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    3.10: Exercise #21 - Blood Agar

    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    THECB - ACGM Lab Learning Outcome #5 Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

    THECB - ACGM Lab Learning Outcome #6 Perform basic bacterial identification procedures using biochemical tests.

    Laboratory Exercise Learning Outcomes
    1. Explain the key ingredients or reagents associated with a blood agar plate.
    2. Define fastidious bacteria and their relation to blood agar plates.
    3. Describe what occurs during each of the following types of hemolysis and how they would appear when utilizing a blood agar plate.
      1. alpha hemolysis
      2. beta hemolysis
      3. gamma hemolysis
    4. Interpret the type of hemolysis for bacterial samples when using a blood agar plate.

    Materials Needed:

    • Bunsen burner
    • Inoculating loop
    • Bacterial cultures (3 different species - instructor will provide name of species to be tested)
    • Blood agar plate (3 plates)
    • Test tube rack


    This page titled 3.10: Exercise #21 - Blood Agar was last modified on Sat, 25 Jul 2026 03:42:53 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.