2.5: Exercise #10 - Structural Stain- Endospore Stain

OER - BIOL 2420 Laboratory Manual · LibreTexts

0%

Contents

    Text

    Size
    100%
    Theme
    Typeface
    Line length
    Spacing

    From LibreTexts, under CC BY 4.0, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #2 Demonstrate proficient use of a compound light microscope.

    THECB - ACGM Lab Learning Outcome #3 Describe and prepare widely used stains and wet mounts and discuss their significance in identification of microorganisms.

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    Laboratory Exercise Learning Outcomes
    1. Explain the difference between a vegetative cell and an endospore.
    2. Describe the function of an endospore.
    3. Name some endospore forming bacteria that are human pathogens and know the diseases they cause.
    4. Explain the steps of the endospore stain.
    5. Perform an endospore stain.
    6. Detect the presence of bacterial endospores.
    7. Distinguish between endospores and vegetative cells in a stained sample.
    8. Explain what information can be gained by performing an endospore stain.
    9. Explain the variations in size, shape, and location of endospores within the vegetative cell.

    Materials Needed:

    • Bunsen burner
    • Microscope slide
    • Staining tray (Containing stains (malachite green, safranin), stain rack, and DI water)
    • China marker
    • Lens cleaner
    • Lens paper
    • Kim wipes
    • Broth culture of bacteria (Bacillus subtilis >72 hours old)
    • Test tube rack
    • Inoculating loop
    • Microscope

    In this laboratory exercise, we will explore the first of two staining techniques that are used to visualize cellular structures, the endospore stain.

    This page titled 2.5: Exercise #10 - Structural Stain- Endospore Stain was last modified on Sat, 25 Jul 2026 03:42:51 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.