4.3.1: Matching

Matching Questions

1. Match the dilution on the left with its correct dilution factor by placing the letter next to the correct dilution.

   Question 1 Terms

   Answer	Terms
   _____	1. 1:100
   _____	2. 1:1,000
   _____	3. 1:10,000
   _____	4. 1:100,000
   _____	5. 1:1,000,000
   _____	6. 1:10,000,000

   Question 1 Descriptions

   Letter	Description
   a.	a dilution factor of 105
   b.	a dilution factor of 102
   c.	a dilution factor of 107
   d.	a dilution factor of 103
   e.	a dilution factor of 106
   f.	a dilution factor of 104

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   Answers:
   1) b
   2) d
   3) f
   4) a
   5) e
   6) c
2. Match the value on the left with the appropriate description of what it refers to by placing the letter next to the correct value.

   Question 2 Terms

   Answer	Terms
   _____	1. 30 - 300
   _____	2. 45 degrees C
   _____	3. 37 degrees C
   _____	4. 24 hours
   _____	5. 25
   _____	6. 0.02 mm cubed
   _____	7. 1,250,000
   _____	8. 2

   Question 2 Descriptions

   Letter	Description
   a.	the temperature at which molten agar is held before it is poured into the petri dish
   b.	the number of large squares etched into each 1 mm squared region of the counting chamber
   c.	the acceptable range of colonies on a plate selected for counting
   d.	the dye dilution factor created when 1 ml of sample is mixed with 1 ml of methylene blue
   e.	the volume of sample present in each 1 mm squared of the counting chamber
   f.	the incubation temperature used for the poured plates
   g.	the dilution factor of the Petroff-Hausser counting chamber
   h.	the length of time the poured plates are incubated before colonies are counted

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   Answers:
   1) c
   2) a
   3) f
   4) h
   5) b
   6) e
   7) g
   8) d
3. Match the procedure step on the left with the reason it is performed by placing the letter next to the correct step.

   Question 3 Terms

   Answer	Terms
   _____	1. Flick the stock culture tube before removing a sample
   _____	2. Hold the melted agar tubes in a 45 degrees C water bath
   _____	3. Gently rotate the plate after the molten agar is poured in
   _____	4. Invert the plates before placing them in the incubator
   _____	5. Add the sample at the edge of the cover slip
   _____	6. Combine 1 ml of sample with 1 ml of methylene blue

   Question 3 Descriptions

   Letter	Description
   a.	moves the agar through the sample until it has been evenly distributed throughout the dish
   b.	suspends the bacteria evenly throughout the media so a representative sample can be taken
   c.	allows the sample to move into the chamber by capillary action
   d.	keeps the agar molten without making it hot enough to kill the bacteria in the sample
   e.	prevents condensation from raining down onto the sample
   f.	stains the cells and creates a 1/2 dilution that must be included in the calculation

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   Answers:
   1) b
   2) d
   3) a
   4) e
   5) c
   6) f