1.3: Exercise #3 - Growth Media and Inoculating Tools

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #5 Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

    Laboratory Exercise Learning Outcomes
    1. Define the difference between defined and complex media and list examples of each.
    2. Describe the difference between liquid, semi-solid, and solid media.
    3. Define and describe the purpose of selective media.
    4. Define and describe the purpose of differential media.
    5. Define and describe the purpose of transport media.
    6. Define and describe the purpose of anaerobic media.
    7. List and describe three tools used for inoculating media.

    Microorganisms require a source of nutrients for growth. In a laboratory setting the nutrients are provided in a form known as media. Media can be classified based on its chemical composition, its physical characteristics, and/or its functional purpose. When introducing microorganisms to a growth medium an inoculating tool is used. Different types of media and methods require different types of inoculating tools. In this laboratory exercise, you will examine growth media based on their chemical classification, physical classification, and functional classification, and three tools used in the microbiology lab for the purpose of inoculation.

    This page titled 1.3: Exercise #3 - Growth Media and Inoculating Tools was last modified on Sat, 25 Jul 2026 03:42:55 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.