3.2: Exercise #13 - Triple Sugar Iron Agar Test

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    THECB - ACGM Lab Learning Outcome #5 Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

    THECB - ACGM Lab Learning Outcome #6 Perform basic bacterial identification procedures using biochemical tests.

    Laboratory Exercise Learning Outcomes
    1. Explain the purpose of the triple sugar iron agar test.
    2. Explain key ingredients or reagents associated with the triple sugar iron agar test.
    3. Interpret the results of the triple sugar iron agar test.

    Materials Needed:

    • Bunsen burner
    • Inoculating loop
    • Agar bacterial cultures (5 different species - instructor will provide name of species to be tested)
    • Triple sugar iron agar tube (5 tubes)
    • Test tube rack

    This page titled 3.2: Exercise #13 - Triple Sugar Iron Agar Test was last modified on Sat, 25 Jul 2026 03:42:51 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.