1.4: Exercise #4 - Aseptic Techniques and Aseptic Transfer of Bacteria

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    THECB - ACGM Lab Learning Outcome #5 Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

    Laboratory Exercise Learning Outcomes
    1. Define aseptic techniques.
    2. Describe the importance of aseptic techniques in the microbiology laboratory.
    3. Demonstrate how to transfer microbes from various types of media using aseptic techniques.

    Materials Needed:

    • Bunsen burner
    • Inoculating loop
    • Test tube rack
    • China marker
    • 1 sharpie marker
    • Simulation broth culture
    • Simulation sterile broth
    • Stock culture (the identity of the bacteria present in the stock culture will be provided by the instructor during the laboratory session)
    • 1 sterile broth tube
    • 1 sterile slant tube
    • 1 Petri dish with nutrient agar

    In this laboratory exercise students will become familiar with aseptic transfer techniques. Microorganisms are ubiquitous, meaning that they are everywhere. Microorganisms are present in the air around us, in and on our bodies, and on the laboratory benches at which you will be working. Therefore, we must use precautionary measures to ensure that our samples do not become contaminated, and we do not become contaminated with them and transport them out of the laboratory. Aseptic transfer techniques are techniques that are used by microbiologists to reduce the likelihood of such contamination.

    This page titled 1.4: Exercise #4 - Aseptic Techniques and Aseptic Transfer of Bacteria was last modified on Sat, 25 Jul 2026 03:42:55 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.