4.2: Exercise #25- Direct Microscopic Count

OER - BIOL 2420 Laboratory Manual · LibreTexts

0%

Contents

    Text

    Size
    100%
    Theme
    Typeface
    Line length
    Spacing

    From LibreTexts, under CC BY 4.0, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions.

    THECB - ACGM Lab Learning Outcome #2 Demonstrate proficient use of a compound light microscope.

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    THECB - ACGM Lab Learning Outcome #7 Estimate the number of microorganisms in a sample using methods such as direct counts, viable plate counts, or spectrophotometric measurements.

    Laboratory Exercise Learning Outcomes
    1. Describe the steps for a direct microscopic count of bacteria.
    2. Demonstrate the ability to determine the number of bacteria in a sample using a direct microscopic count.

    Materials Needed:

    • Test tube rack
    • E. coli culture (16-24 hours old)
    • 1 mL pipette & Pipette pump or micropipettor with tips
    • Methylene blue dye
    • Test tube
    • Microscope
    • Immersion oil

    This page titled 4.2: Exercise #25- Direct Microscopic Count was last modified on Sat, 25 Jul 2026 03:42:53 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.