3.13.2: Fill-In-The-Blank

1. An organism inoculated onto blood agar and observed to produce a clear zone surrounding the bacterial growth following incubation, this is known as hemolysis.

2. Catalase breaks down into water and oxygen gas.

3. The indicator used in the carbohydrate fermentation test is .

4. An organism capable of producing citrate permease (citrase) will cause Simmons citrate media to turn .

5. The pH indicator used in the urease test is .

6. The test determines a bacterium’s ability to produce the enzyme amylase.

7. The end product of the nitrate reductase reaction is .

8. The addition of hydrogen peroxide to bacterial sample to detect gas bubble formation is done in the test.

9. The pH indicator neutral red used in MacConkey agar will cause a color change to when acidic conditions from fermentation of lactose occur.

10. The milk protein found in milk that makes it white and cloudy is .

11. Mannitol salt agar is selective for salt tolerant bacteria, which are properly referred to as .

12. The presence of starch is determined by a dark-blue/purple color when is added.

13. is the enzyme that reduces oxygen in the electron transport chain.

14. The test determines a bacterium’s ability to produce an enzyme that permits it to clot blood thereby enhancing its virulence.

15. The test determines the ability of a bacterium to produce the enzyme tryptophanase.

16. The test is used to differentiate Staphylococci from Streptococci on the basis of their ability to produce the enzyme necessary to reduce hydrogen peroxide to water and oxygen gas.

17. The test determines the ability of a bacterium to produce hydrogen sulfide gas its ability to produce the enzyme tryptophanase, and if it is capable of motility.

18. A bacterium capable of producing hydrogen sulfide gas will turn the color of the media at the end of a triple sugar iron agar test.

19. A mannitol fermenting facultative halophile will cause the media of the mannitol salt agar to change in color to .

20. reagent is used to detect the presence of indole in both the SIM agar test and the indole test.

21. The pH indicator used in the MacConkey agar is .

22. The tests included in the IMViC panel of tests in order of their abbreviations are the

23. The test determines a bacterium’s ability to produce the enzyme needed at the end of the electron transport chain to reduce molecular oxygen to water.

24. A positive catalase test is determined by the production of after the addition of hydrogen peroxide to a bacterial sample.

25. The enzymes that allow bacteria to lysis red blood cells on blood agar are called .