4.1: Exercise #24 - Viable Plate Counts

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    From LibreTexts, under CC BY 4.0, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    THECB - ACGM Lab Learning Outcome #5 Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

    THECB - ACGM Lab Learning Outcome #7 Estimate the number of microorganisms in a sample using methods such as direct counts, viable plate counts, or spectrophotometric measurements.

    Laboratory Exercise Learning Outcomes
    1. Define serial dilution.
    2. Define colony and colony forming unit.
    3. Describe the steps involved in performing a serial dilution and viable plate count.
    4. Demonstrate the ability to calculate serial dilutions.
    5. Demonstrate the ability to determine the number of bacteria in a sample using serial dilutions and viable plate counts.

    Materials Needed:

    • Test tube rack
    • E. coli culture (24-48 hours old)
    • 7 sterile water blank tubes
    • 6 nutrient agar deep tubes
    • Water bath (45°C)
    • Sterile mL serological pipettes (11)
    • Pipette pump
    • Biohazard container for sharps

    This page titled 4.1: Exercise #24 - Viable Plate Counts was last modified on Sat, 25 Jul 2026 03:42:53 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.