3.7.2: Coagulase Test Activity

In this activity you will test two bacterial samples. Label each coagulase tube with the sample it is to be inoculated with.

1. Turn on the Bunsen burner and sterilize the inoculating loop by passing it through the flame of the burner.
2. Obtain a bacterial culture. Remove the cap. Pass the mouth of the tube through the flame of the Bunsen burner.
3. Insert the loop into the tube to acquire a sample of the bacterium.
4. Pass the tube through the flame of the Bunsen burner and recap the test tube, and return the tube to the test tube rack.
5. Pick up the rabbit plasma tube to which the sample is to be transferred. Remove the cap and pass the mouth of the tube through the flame of the Bunsen burner.
6. Insert the loop into the tube to introduce the bacterial sample to the media.
7. Pass the mouth of the rabbit plasma tube through the flame and recap it, and return it to the test tube rack.
8. Sterilize the inoculating loop by flaming the loop in the flame of the Bunsen burner.

Repeat steps 1 - 8 for the second bacterial species and rabbit plasma tubes to be inoculated.

9. Incubate the inoculated coagulase tubes at 37°C for 24 hours.
10. After 24 hours observe the tube for evidence of clot formation, any clumping.

In the table below write the name of the species tested and the results for each of the tests performed.

Experimental Results

Bacterial Species	Appearance of media following incubation	Coagulase Test Results

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