3.12: Exercise #23 - Mannitol Salt Agar

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    From LibreTexts, under CC BY 4.0, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    THECB - ACGM Lab Learning Outcome #5 Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

    THECB - ACGM Lab Learning Outcome #6 Perform basic bacterial identification procedures using biochemical tests.

    Laboratory Exercise Learning Outcomes
    1. Explain the purpose of mannitol salt agar.
    2. Describe the ingredients that make mannitol salt agar a selective media.
    3. Describe why mannitol salt agar is considered a selective media
    4. Describe the ingredients that make mannitol salt agar a differential media.
    5. Describe why the mannitol salt agar is considered a differential media.
    6. Interpret the results when observing bacterial growth on mannitol salt agar.

    Materials Needed:

    • Bunsen burner
    • Inoculating loop
    • Bacterial cultures (2 different species - instructor will provide name of species to be tested)
    • Mannitol salt agar plate (2 plates)
    • Test tube rack

    This page titled 3.12: Exercise #23 - Mannitol Salt Agar was last modified on Sat, 25 Jul 2026 03:42:53 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.