2.7.1: Matching

Matching Questions

1. Match the cell type or structure to the appropriate description of its appearance following staining by placing the letter next to the correct term.

   Question 1 Terms

   Answer	Term
   _____	1. Acid-fast cells
   _____	2. Non-acid-fast cells
   _____	3. Gram-positive cell
   _____	4. Gram-negative cell
   _____	5. Endospore
   _____	6. Vegetative cell (mother cell)
   _____	7. Capsule

   Question 1 Descriptions

   Letter	Description
   a.	purple due to the presence of crystal violet and iodine
   b.	pink due to the presence of safranin after decolorization with water
   c.	pink due to the presence of safranin after decolorization with acetone and/or alcohol
   d.	clear halo surrounding a cell
   e.	blue due to presence of methylene blue
   f.	red due to the presence of carbolfuchsin
   g.	green due to the presence of malachite green

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   Answers:
   1-f
   2-e
   3-a
   4-c
   5-g
   6-b
   7-d
2. Match the staining procedure on the left with their appropriate descriptions by placing the letter next to the correct term.

   Question 2 Staining Procedures

   Answer	Staining Procedure
   _____	1. Gram stain
   _____	2. Acid-fast stain
   _____	3. Endospore stain
   _____	4. Capsule stain
   _____	5. Simple stain
   _____	6. Negative stain

   Question 2 Descriptions

   Letter	Description
   a.	staining procedure that makes use of a single dye; allows one to determine a bacterium’s shape, size, and arrangement
   b.	staining procedure used to detect the presence of mycolic acid
   c.	staining procedure in which only an acidic dye is applied to the sample
   d.	staining procedure that separates bacteria into two groups based on difference in their cell wall structure/thickness
   e.	staining procedure that uses both an acidic and basic stain to detect a form of glycocalyx
   f.	staining procedure used to detect the formation of metabolically inactive structures produced by a few genera of bacteria

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   Answers:
   1-d
   2-b
   3-f
   4-e
   5-a
   6-c
3. Match the terms regarding bacterial shapes or arrangements on the left with their appropriate descriptions by placing the letter next to the correct term.

   Question 3 Terms

   Answer	Term
   _____	1. Coccus
   _____	2. Bacillus
   _____	3. Palisade
   _____	4. Streptococcus
   _____	5. Streptobacillus
   _____	6. Staphylococcus
   _____	7. Spirillum
   _____	8. Spirochete
   _____	9. Vibrio
   _____	10. Pleomorphic

   Question 3 Descriptions

   Letter	Description
   a.	spiral shaped bacterium with rigid cell wall
   b.	chain of bacillus cells
   c.	spherical shaped bacterial cells
   d.	chain of spherical cells
   e.	term used for bacteria that exhibit a variety of shapes and sizes
   f.	clusters of spherical cells
   g.	rod shaped bacterial cells
   h.	spiral shaped bacterium with flexible cell walls
   i.	rod shaped bacteria arranged in a pattern parallel to one another
   j.	curved rods

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   Answers:
   1-c
   2-g
   3-i
   4-d
   5-b
   6-f
   7-a
   8-h
   9-j
   10-e