5.4: Exercise #29 - Antimicrobial Sensitivity Test (Kirby-Bauer Method)

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    THECB - ACGM Lab Learning Outcome #5 Use different types of bacterial culture media to grow, isolate, and identify microorganisms.

    Laboratory Exercise Learning Outcomes
    1. Describe the importance of Mueller-Hinton agar to the antimicrobial sensitivity test (Kirby-Bauer method).
    2. Define zone of inhibition.
    3. Define antimicrobial resistance.
    4. Describe how an antimicrobial sensitivity test is performed.
    5. Describe how antimicrobials are classified as susceptible (S), intermediate (I), or resistant (R), following and antimicrobial sensitivity test.
    6. Correctly interpret a Kirby-Bauer plate to determine if an organism is susceptible, intermediate, or resistant to an antimicrobial agent.
    7. Describe the clinical importance of an antimicrobial sensitivity test (Kirby-Bauer method).

    Materials Needed:

    • Bunsen burner
    • 3 Mueller-Hinton agar plates
    • 4-6 hour old cultures of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae
    • 4 sterile cotton swabs
    • Antibiotic disks
    • Antibiotic disk dispenser or 70% ethanol in beakers and forceps
    • Metric rulers

    This page titled 5.4: Exercise #29 - Antimicrobial Sensitivity Test (Kirby-Bauer Method) was last modified on Sat, 25 Jul 2026 03:42:56 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.