2.6: Exercise #11 - Structural Stain- Capsule Stain

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    Exercise Outcomes:

    Course Laboratory Learning Outcomes

    THECB - ACGM Lab Learning Outcome #1 Use and comply with laboratory safety rules, procedures, and universal precautions

    THECB - ACGM Lab Learning Outcome #2 Demonstrate proficient use of a compound light microscope.

    THECB - ACGM Lab Learning Outcome #3 Describe and prepare widely used stains and wet mounts and discuss their significance in identification of microorganisms.

    THECB - ACGM Lab Learning Outcome #4 Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.

    Laboratory Exercise Learning Outcomes
    1. Describe what capsules are.
    2. Explain how bacterial cells benefit from capsules.
    3. Provide some examples of pathogens that have capsules.
    4. Explain why the capsule stain is referred to as a negative staining technique.
    5. Describe the protocol followed to stain for capsules.
    6. Perform a capsule stain.

    Materials Needed:

    • Bunsen burner
    • Microscope slides (one for each bacterial sample)
    • Staining tray (Containing stains (congo red, Maneval's stain), stain rack, and DI water)
    • China marker
    • Lens cleaner
    • Lens paper
    • Kim wipes
    • Broth culture of bacteria
    • Test tube rack
    • Inoculating loop
    • Microscope

    In this laboratory exercise, we will explore the second of two staining techniques that are used to visualize cellular structures, the capsule stain.

    This page titled 2.6: Exercise #11 - Structural Stain- Capsule Stain was last modified on Sat, 25 Jul 2026 03:42:51 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.