3.4: Exercise 15 - IMViC Tests

OER - BIOL 2420 Laboratory Manual · LibreTexts

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    Exercise Outcomes

    Course Laboratory Learning Outcomes
    • THECB - ACGM Lab Learning Outcome #1: Use and comply with laboratory safety rules, procedures, and universal precautions.
    • THECB - ACGM Lab Learning Outcome #4: Perform basic microbiology procedures using aseptic techniques for transfer, isolation, and observation of commonly encountered, clinically significant bacteria.
    • THECB - ACGM Lab Learning Outcome #5: Use different types of bacterial culture media to grow, isolate, and identify microorganisms.
    • THECB - ACGM Lab Learning Outcome #6: Perform basic bacterial identification procedures using biochemical tests.
    Laboratory Exercise Learning Outcomes
    1. List the tests that are part of an IMViC panel of tests.
    2. Explain what group of bacteria these tests aid in identifying.
    3. Explain the purpose of the indole test.
    4. Explain key ingredients or reagents associated with the indole test.
    5. Interpret the results of the indole test.
    6. Explain the purpose of the methyl red test.
    7. Explain key ingredients or reagents associated with the methyl red test.
    8. Interpret the results of the methyl red test.
    9. Explain the purpose of the Voges-Proskauer test.
    10. Explain key ingredients or reagents associated with the Voges-Proskauer test.
    11. Interpret the results of the Voges-Proskauer test.
    12. Explain the purpose of the citrate test.
    13. Explain key ingredients or reagents associated with the citrate test.
    14. Interpret the results of the citrate test.

    IMViC Tests

    The IMViC tests are a panel of tests that include the indole test (I), the methyl red test (M), the Voges-Proskauer test (Vi), and the citrate test (C). These tests are performed in order to differentiate members of the Enterobacteriaceae family.

    Members of this family are commonly known as enterobacteria or enteric bacteria. This is because many members of this family reside within the intestines of humans or animals. The enterobacteria are gram-negative bacilli. While morphologically very similar, they can be distinguished by their biochemical properties. While most members of this family are not pathogenic, some do act as pathogens. Some medically important members of this family include bacteria such as Klebsiella spp., Shigella spp., Salmonella spp., and Escherichia coli.

    This page titled 3.4: Exercise 15 - IMViC Tests was last modified on Sat, 25 Jul 2026 03:42:52 GMT and is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Sherry Smith.