2.6.2: Capsule Stain Activity

In this activity you will prepare one bacterial sample using the capsule stain procedure.

1. Obtain two microscope slides, clean and dry the microscope slide using lens cleaner and Kim wipes
2. Label the slides using a China marker as seen below

   ***NOTE*** the circle must be placed on the bottom of the slide to prevent the marker from being mixed with the bacterial sample

   

3. Place a drop of Congo Red on the slides
4. With an inoculating loop, transfer a loopful of inoculum to the Congo Red on each slide; Mix and spread out the dye-culture mixture into a thin even film using a clean microscope slide. Set the slides aside and let the slides air dry completely. Do not heat fix!!!
5. When the slides are dry, apply Maneval’s stain to the dried films on the slides so that they are completely covered and let stand for 1-2 minutes
6. Pour off the excess stain, wash the slides very gently with deionized water
7. Use Kim wipes to blot the slides, smear side down. Blot, do not rub!!!!
8. Use your compound microscope to visualize your stained slides.

   

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