Bio 221Lab: Introduction to Microbiology

by Kelly C. Burke

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This lab manual provides an introduction to the biology and ubiquity of microorganisms (for example, bacteria, viruses, protozoa, fungi), including morphology, anatomy, physiology, classification, and identification. The significance and role of microorganisms in human affairs will also be explored.

Description from LibreTexts.

Publisher
College of the Canyons
Subjects
Biology
Licence
CC BY, as LibreTexts states it
Who can read
Tweens profiles and older, judged from its title and subjects

Contents

LibreTexts writes equations in TeX, and here they show as TeX; the PDF has them typeset.

  1. Front Matter
  2. TitlePage
  3. InfoPage
  4. Table of Contents
  5. Licensing
  6. Unit 1: Safety
  7. 1.1: Learning Objectives
  8. 1.2: Introduction
  9. 1.3: Materials and Procedures
  10. 1.4: Post-lab Questions
  11. 1.5: Conclusion and Resources
  12. Unit 2: The Metric System, Measurement, and Lab Equipment Review
  13. 2.1: Learning Objectives
  14. 2.2: Introduction
  15. 2.3: Pre-lab Questions
  16. 2.4: Materials and Procedures
  17. 2.5: Post-lab Questions
  18. 2.6: Conclusion and Resources
  19. Unit 3: Microscopy
  20. 3.1: Learning Objectives
  21. 3.2: Introduction
  22. 3.3: Pre-lab Questions
  23. 3.4: Materials and Procedures
  24. 3.5: Post-lab Questions
  25. 3.6: Conclusion and Resources
  26. Unit 4: Environmental Sampling
  27. 4.1: Learning Objectives
  28. 4.2: Introduction
  29. 4.3: Pre-lab Questions
  30. 4.4: Materials and Procedures
  31. 4.5: Post-lab Questions
  32. 4.6: Conclusion and Resources
  33. Unit 5: Survey of Eukaryotic Microorganisms- The Protists Algae
  34. 5.1: Learning Objectives
  35. 5.2: Introduction to Protists
  36. 5.3: Pre-lab Questions
  37. 5.4: Materials and Procedures
  38. 5.5: Post-lab Questions
  39. 5.6: Conclusion and Resources
  40. Unit 6: Parasitic Helminths
  41. 6.1: Learning Objectives
  42. 6.2: Introduction
  43. 6.3: Pre-lab Questions
  44. 6.4: Materials and Procedures
  45. 6.5: Post-lab Questions
  46. 6.6: Conclusion and Resources
  47. Unit 7: Fungi
  48. 7.1: Learning Objectives
  49. 7.2: Introduction
  50. 7.3: Pre-lab Questions
  51. 7.4: Materials and Procedures
  52. 7.5: Post-lab Questions
  53. 7.6: Conclusion and Resources
  54. Unit 8: Pure Cultures- Aseptic Transfer Techniques and Streak Plates for Isolation
  55. 8.1: Learning Objectives
  56. 8.2: Introduction
  57. 8.3: Pre-lab Questions
  58. 8.4: Materials and Procedures
  59. 8.5: Post-lab Questions
  60. 8.6: Conclusion and Resources
  61. Unit 9: Bacterial Growth Patterns- Building your Stock Cultures and Observing Culture Characteristics
  62. 9.1: Learning Objectives
  63. 9.2: Introduction
  64. 9.3: Pre-lab Questions
  65. 9.4: Materials and Procedures
  66. 9.5: Post-lab Questions
  67. 9.6: Conclusion and Resources
  68. Unit 10: Bacterial Growth Patterns- Direct Count, The Standard Plate Count, and Indirect Turbidimetric Methods
  69. 10.1: Learning Objectives
  70. 10.2: Introduction
  71. 10.3: Pre-lab Questions
  72. 10.4: Materials and Procedures
  73. 10.5: Post-lab Questions
  74. 10.6: Conclusion and Resources
  75. Unit 11: Environmental Effects on Growth- Temperature
  76. 11.1: Learning Objectives
  77. 11.2: Introduction
  78. 11.3: Pre-lab Questions
  79. 11.4: Materials and Procedures
  80. 11.5: Post-lab Questions
  81. 11.6: Conclusion and Resources
  82. Unit 12: Environmental Effects on Growth- pH
  83. 12.1: Learning Objectives
  84. 12.2: Introduction
  85. 12.3: Pre-lab Questions
  86. 12.4: Materials and Procedures
  87. 12.5: Post-lab Questions
  88. 12.6: Conclusion and Resources
  89. Unit 13: Environmental Effects on Growth- Osmotic Pressure
  90. 13.1: Learning Objectives
  91. 13.2: Introduction
  92. 13.3: Pre-lab Questions
  93. 13.4: Materials and Procedures
  94. 13.5: Post-lab Questions
  95. 13.6: Conclusion and Resources
  96. Unit 14: Oxygen Requirements- FTM and the Anaerobe Jar
  97. 14.1: Learning Objectives
  98. 14.2: Introduction
  99. 14.3: Pre-lab Questions
  100. 14.4: Materials and Procedures
  101. 14.5: Post-lab Questions
  102. 14.6: Conclusion and Resources
  103. Unit 15: Environmental Effects on Growth- Antimicrobial Sensitivity Testing
  104. 15.1: Learning Objectives
  105. 15.2: Introduction
  106. 15.3: Pre-lab Questions
  107. 15.4: Materials and Procedures
  108. 15.5: Post-lab Questions
  109. 15.6: Conclusion and Resources
  110. Unit 16: Transformation\(^1\)
  111. 16.1: Learning Objectives
  112. 16.2: Introduction
  113. 16.3: Pre-lab Questions
  114. 16.4: Materials and Procedures
  115. 16.5: Post-lab Questions
  116. 16.6: Conclusion and Resources
  117. Unit 17: Smear Prep and Simple Stains
  118. 17.1: Learning Objectives
  119. 17.2: Introduction
  120. 17.3: Pre-lab Questions
  121. 17.4: Materials and Procedures
  122. 17.5: Post-lab Questions
  123. 17.6: Conclusion and Resources
  124. Unit 18: Negative Stain
  125. 18.1: Learning Objectives
  126. 18.2: Introduction
  127. 18.3: Pre-lab Questions
  128. 18.4: Materials and Procedures
  129. 18.5: Post-lab Questions
  130. 18.6: Conclusion and Resources
  131. Unit 19: Gram Stain
  132. 19.1: Learning Objectives
  133. 19.2: Introduction
  134. 19.3: Pre-lab Questions
  135. 19.4: Materials and Procedures
  136. 19.5: Post-lab Questions
  137. 19.6: Conclusion and Resources
  138. Unit 20: Endospore Stain
  139. 20.1: Learning Objectives
  140. 20.2: Introduction
  141. 20.3: Pre-lab Questions
  142. 20.4: Materials and Procedures
  143. 20.5: Post-lab Questions
  144. 20.6: Conclusion and Resources
  145. Unit 21: Acid-Fast Stain- Kinyoun Method
  146. 21.1: Learning Objectives
  147. 21.2: Introduction
  148. 21.3: Pre-lab Questions
  149. 21.4: Materials and Procedures
  150. 21.5: Post-lab Questions
  151. 21.6: Conclusions and Resources
  152. Unit 22: Physiological Tests for Characterization and Identification of Bacteria
  153. 22.1: Learning Objectives
  154. 22.2: Selective and Differential Media - MacConkey, EMB, MSA
  155. 22.3: Chromogenic Media
  156. 22.4: Blood Agar Plates (BAP)
  157. 22.5: Fermentation and Utilization Media-Durham Sugar Tubes, MRVP, Oxidase, Catalase, Citrate
  158. 22.6: Hydrolytic and Miscellaneous Media - Starch, Skim Milk, Gelatin, Indole, Urea, Kligler’s/TSI
  159. Unit 23: Unknown 1 - What is yellow, wrinkled, round,...?
  160. 23.1: Learning Objectives
  161. 23.2: Introduction
  162. 23.3: Pre-lab Questions
  163. 23.4: Materials and Procedures
  164. 23.5: Conclusion and Resources
  165. Unit 24: Unknown 2- Mixed Culture
  166. 24.1: Learning Objectives
  167. 24.2: Introduction
  168. 24.3: Pre-lab Questions
  169. 24.4: Materials and Procedures
  170. 24.5: Conclusion and Resources
  171. Unit 25: Bacterial Examination of Food- Standard Plate Counts
  172. 25.1: Learning Objectives
  173. 25.2: Introduction
  174. 25.3: Pre-lab Questions
  175. 25.4: Materials and Procedures
  176. 25.5: Post-lab Questions
  177. 25.6: Conclusion and Resources
  178. Unit 26: Bacterial Examination of Water- Multiple Tube Test, Standard Plate Count, and Membrane Filter Technique
  179. 26.1: Learning Objectives
  180. 26.2: Introduction
  181. 26.3: Pre-lab Questions
  182. 26.4: Materials and Procedures
  183. 26.5: Post-lab Questions
  184. 26.6: Conclusion and Resources
  185. Unit 27: Immunology- ELISA-Simulation, StaphTEX-Agglutination Reaction
  186. 27.1: Learning Objectives
  187. 27.2: Introduction
  188. 27.3: Materials and Procedures
  189. 27.4: Post-lab Questions
  190. 27.5: Conclusion and Resources
  191. Unit 28: Microbescopes and Observation of Natural Samples
  192. 28.1: Learning Objectives
  193. 28.2: Introduction
  194. 28.3: Pre-lab Questions
  195. 28.4: Materials and Procedures
  196. 28.5: Post-lab Questions
  197. 28.6: Conclusion and Resources
  198. Back Matter
  199. Index
  200. Glossary
  201. Detailed Licensing