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14.4: Materials and Procedures

Bio 221Lab: Introduction to Microbiology · LibreTexts

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    From LibreTexts, under CC BY, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    Materials

    • 3 FTM tubes
    • 2 TSA plates
    • GasPak jar, sachets, indicator
    • Bacterial cultures:
      • Staphylococcus saprophyticus (Use student stock organism)
      • Pseudomonas fluorescens (Use student stock organism)
      • Clostridium sporogenes (Use slant on front table, one per table only)

    Procedures

    1. Quickly streak each of the 3 organisms onto 2 TSA plates:
    Simple line drawing of an abstract face inside a circular outline.
    1. Immediately place 1 plate in the anaerobe jar. Incubate the other plate aerobically in your class incubator.
    2. Inoculate each organism into a separate Fluid Thioglycolate Medium(FTM):

    Staphylococcus saprophyticus, Pseudomonas fluorescens, Clostridium sporogenes

    1. Incubate FTM tubes in your class incubator.

    Results

    1. GasPak: Compare the GasPak plate to the plate incubated aerobically and fill in the following chart.

    Organism

    GasPak growth (+/-)

    Aerobic growth (+/-)

    Oxygen requirement type

    1. FTM: Draw your FTM tubes and label each with the appropriate oxygen requirement type

    This page titled 14.4: Materials and Procedures was last modified on Sat, 16 May 2026 01:43:52 GMT and is shared under a CC BY license and was authored, remixed, and/or curated by Kelly C. Burke.