5.4: Materials and Procedures

Bio 221Lab: Introduction to Microbiology · LibreTexts

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    From LibreTexts, under CC BY, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    Materials

    Wet Mount Materials:

    • Glass slides
    • Hanging drop slides
    • Coverslips
    • Vaseline
    • Detain/Protoslo

    Live Material:

    • Mixed Protists

    Prepared Slides:

    • Amoeba proteus
    • Trypanosoma gambiense (West African trypanosomiasis)
    • Plasmodium vivax (benign tertian malaria)
    • Paramecium caudatum
    • Giardia lamblia (giardiasis)
    • Cryptospiridium parvum (gastrointestinal disease)
    • Entamoeba histolytica (Amoebic dysentery)
    • Trichomonas vaginalis (STD)

    Procedures

    1. Live material: Observe the live Mixed Protist Survey via a wet mount or hanging drop slide. View the specimen with Bright Field, Phase Contrast, and Dark Field if your microscope is equipped with it. Draw what you observe.
    Illustration comparing "Wet Mount" left and "Hanging Drop" right, each showing slides with blue and gray droplets.
    1. Prepared slides: Examine the prepared slides (use Bright Field only). Refer to images provided in order to help you find the correct organism/structure.
    2. Review the life cycles for each disease. Make note of habitat, hosts, transmission routes, and specialized structures.

    Results

    Draw the organisms as you see them through the microscope. Record the total magnification and a measurement. Indicate on the drawing what you measured with a bar symbol: \(\mid -\mid \)

    Four blank circles arranged in a 2x2 grid, each with spaces for "Chapter," "Title," "Magnifier," and "Magnification."

    Four blank circles with spaces beneath each for "Chapter," "Title," "Magnitude," and "Magnitude Number."

    Four blank circles arranged in two rows, each with labeled sections for "Chapter," "Title," and "Magnifier."

    This page titled 5.4: Materials and Procedures was last modified on Sat, 16 May 2026 01:42:23 GMT and is shared under a CC BY license and was authored, remixed, and/or curated by Kelly C. Burke.