6.4: Materials and Procedures

Materials

Wet Mount Materials:

• Glass slides
• Coverslips

Live Material:

• Turbatrix aceti- Vinegar Eels (Nematoda)

Preserved Materials:

• Flukes and Tapeworms
• Dissected Ascaris (Unsegmented Roundworms)
• Prepared Slides:
• Phylum Platyhelminthes: (on back bench)
• Class Trematoda (Flukes)
• Fasciola hepatica (liver flukes)
• Clonorchis sinensis (Oriental Liver Fluke)
• Schistosoma sp. (Blood flukes)
• Class Cestoda (segmented Flatworms)
• Taenia sp. (proglotids) (Tapeworm)
• Dypilidium caninum (Dog tapeworm)
• Phylum Nematoda (unsegmented Roundworms):
• Ascaris lumbricoides
• Enterobius vermicularis (Pinworm)
• Necator americanus (Hookworm)
• Trichinella spiralis

Procedures

1. Wet Mount of Turbatrix aceti (Nematoda):
   1. Place one drop of sample onto a cleaned slide. Add a drop of Detain if desired to slow down the movement of the nematode.
   2. Carefully place a coverslip over sample.
   3. Observe under the microscope (low and high power) with bright-field, dark-field, and phase contrast. Draw some of your observations.
   4. Turbatrix can also be observed via a dissecting scope.
2. Prepared specimens: Observe and draw.
3. Prepared slides: Observe, draw, label, and measure the helminths and their structures. Pay close attention to the label on each slide. The slide will give hints as to what you will observe. Terms/abbreviations that you may see on the label:

WM=Whole Mount-the entire organism is on the slide

CS=Cross Section-the organism has been cut across the body in the vertical plane and is mounted on end. You are looking through a cross section of the organism. Think of making orange slices and looking through the cut segments.

TS=Transverse Section

LS=Longitudinal Section-cuts across the horizontal plane.

Results