12.4: Materials and Procedures

Bio 221Lab: Introduction to Microbiology · LibreTexts

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    From LibreTexts, under CC BY, as the source states it; its equations are written in TeX, which the PDF shows typeset. This page at LibreTexts.

    Materials

    2 Groups per Table:

    • Organisms used from the Student Stock Cultures(TSB):
      • Staphylococcus saprophyticus
      • Escherichia coli
      • Alcaligenes viscolactis
      • Lactococcus lactis
    • 4 tubes: TSB pH 4.0
    • 4 tubes: TSB pH 6.0
    • 4 tubes: TSB pH 8.0
    • 4 tubes: TSB pH 10.0

    Procedures

    Each Group:

    1. Use a sterile transfer pipette to inoculate each of the 4 organisms into each pH tube:
      • add 1 drop of broth culture to each pH broth tube.
      • Staphylococcus saprophyticus → TSB pH 4.0, pH 6.0, pH 8.0, pH 10.0
      • Escherichia coli → TSB pH 4.0, pH 6.0, pH 8.0, pH 10.0
      • Alcaligenes viscolactis → TSB pH 4.0, pH 6.0, pH 8.0, pH 10.0
      • Lactococcus lactis → TSB pH 4.0, pH 6.0, pH 8.0, pH 10.0
    2. Place the tubes in buckets and incubate in your classroom incubator.

    Results

    Enter your ratings for each bacterium/pH and give the pH classification for bacterium.

    Organism

    pH 4

    pH 6

    pH 8

    pH 10

    Optimum pH

    pH Classification

    This page titled 12.4: Materials and Procedures was last modified on Sat, 16 May 2026 01:32:09 GMT and is shared under a CC BY license and was authored, remixed, and/or curated by Kelly C. Burke.