Learn · NPTEL

Course10210105028 lectures

Mass spectrometry based proteomics

Prof. Sanjeeva Srivastava · IIT Bombay

Start with lecture 1 At NPTEL

Lectures

Week 1: Proteomics introduction and sample preparation

  1. Introductory lecture Video
  2. Lecture 1: Introduction to proteomics Video
  3. Lecture 2: Proteomics and sample preparation Video
  4. Lecture 3: Bacterial protein extraction Video
  5. Lecture 4: In-gel digestion Video
  6. Lecture 5: Fundamentals of mass spectrometry Video
  7. Lab session 1.1 : Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR Video
  8. Lab Session 1.2: Demonstration of Q-TOF MS technology Video

Week 2 : Basics of mass spectrometry

  1. Lecture 6: Chromatography technologies Video
  2. Lecture 7: Liquid chromatography Video
  3. Lecture 8: Mass spectrometry: Ionization sources Video
  4. Lecture 9: Mass spectrometry: Mass analyzers Video
  5. Lecture 10: MALDI sample preparation and analysis Video
  6. Lab session 2.1: Global proteomics - experimental design and sample preparation Video
  7. Lab session 2.2: Global proteomics - instrumentation and data acquisition Video

Week 3: Quantitative proteomics

  1. Lecture 11: Introduction to quantitative proteomics Video
  2. Lecture 12: Hybrid mass spectrometry configurations Video
  3. Lecture 13: SILAC: In Vivo labeling Video
  4. Lecture 14: iTRAQ: In Vitro labeling Video
  5. Lecture 15: TMT: In vitro labeling Video
  6. Lab session 3.1: Targeted proteomics - experimental design Video
  7. Lab session 3.2: Targeted proteomics - sample preparation, data acquisition and data analysis using skyline Video

Week-4: Proteomics and systems biology

  1. Lecture 16: Quantitative proteomics data analysis Video
  2. Lecture 17: Proteomics and Systems biology I Video
  3. Lecture 18: Proteomics & Systems biology II Video
  4. Lecture 19: Proteomics applications Video
  5. Lecture 20: Advances and challenges in proteomics Video
  6. Lab session 4.1: Protein-protein interaction using label-free biosensors Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Bombay
Department
Biotechnology
Teacher
Prof. Sanjeeva Srivastava
Level
Undergraduate
Language
English
Subjects
Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
Who can watch
NPTEL gives no rating. Invisible Network shows it to Tweens profiles and older, judged from what the source says about it. How courses are placed