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Course10210105440 lectures

Interactomics

Prof. Sanjeeva Srivastava · IIT Bombay

Start with lecture 1 At NPTEL

Lectures

Week 1

  1. Lecture 1 : Introduction to Interactomics Video
  2. Lecture 2 : An overview of label-free technologies Video
  3. Lecture 3 : An overview of surface plasmon resonance (SPR) Video
  4. Lecture 4 : An overview of surface plasmon resonance imaging (SPRi) Video
  5. Lecture 5 : Basics of SPR: Surface chemistry Video

Week 2

  1. Lecture 6 : Basics of SPR: Experimental design Video
  2. Lecture 7 : Protein immobilization for protein-protein interaction studies Video
  3. Lecture 8 : Protein-protein interaction study: Binding analysis Video
  4. Lecture 9 : Protein-protein interaction study: Kinetic analysis Video
  5. Lecture 10 : Protein-small molecule interaction study: Immobilization & binding analysis Video

Week 3

  1. Lecture 11 : Protein-small molecule interaction study: Kinetic analysis Video
  2. Lecture 12 : SPR: Interactive Session - I Video
  3. Lecture 13 : SPR: Interactive Session - II Video
  4. Lecture 14 : An overview of ellipsometry and interferometry techniques Video
  5. Lecture 15 : An introduction to BioLayer Interferometry (BLI) and its applications in protein research Video

Week 4

  1. Lecture 16 : Kinetic analysis of protein-protein interaction using BLI Video
  2. Lecture 17 : Label-free quantification of proteins using BLI Video
  3. Lecture 18 : Diffraction-based biosensors - I Video
  4. Lecture 19 - Diffraction-based biosensors - II Video
  5. Lecture 20 - Nanotechniques in proteomics - I Video
  6. Lecture 21 - Nanotechniques in proteomics - II Video

Week 5

  1. Lecture 22 : High throughputplatforms of interactomics: Protein arrays Video
  2. Lecture 23 : Conventional label based detection techniques for Protein microarrays Video
  3. Lecture 24 : Novel detection techniques for Protein microarrays Video
  4. Lecture 25 : Recombinational cloning and its application for Protein microarrays Video
  5. Lecture 26 : An introduction to Cell-free protein synthesis Video

Week 6

  1. Lecture 27 : Cell-free synthesis based protein microarrays: PISA and NAPPA Video
  2. Lecture 28 : Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays Video
  3. Lecture 29 : Digging deeper into NAPPA: Basic Workflow Video
  4. Lecture 30 : Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment Video
  5. Lecture 31 : Application of cell free expression protein microarrays in biomarker discovery Video

Week 7

  1. Lecture 32 : Application of cell-free expression protein microarrays in immunological studies Video
  2. Lecture 33 : Basics of microarray image scanning Video
  3. Lecture 34 : Software for Image scanning and data processing Video
  4. Lecture 35 : Microarray Data Analysis : Part I Video
  5. Lecture 36 : Microarray Data Analysis : Part II Video

Week 8

  1. Lecture 37 : Application of protein microarray in biomarker discovery-I Video
  2. Lecture 38 : Application of protein microarray in biomarker discovery-II Video
  3. Lecture 39 : Systems biology and networks Video
  4. Lecture 40 : Challenges in proteomics Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Bombay
Department
Biotechnology
Teacher
Prof. Sanjeeva Srivastava
Level
Undergraduate
Language
English
Subjects
Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
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