Course10210105540 lectures
Introduction to Proteomics
Prof. Sanjeeva Srivastava · IIT Bombay
About
This course introduces to the basic biology of proteins and the new advanced science called as proteomics which aims to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. The course will cover in detail the two major aspects of proteomics i.e., Gel-based proteomics and Mass spectrometry-based proteomics. The gel-based module will cover different techniques like SDS-PAGE, 2-DE, 2D-DIGE etc. These techniques had a major contribution in transition from protein chemistry to proteomics. Mass spectrometry, on the other hand, is an advanced analytical technique for accurate mass measurement. In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics.
INTENDED AUDIENCE : It would be applied to B.Sc., M.Sc. and MS.
PRE-REQUISITES : Any B.Sc. Or M.Sc. The target audiences of this course are required to have a basic introduction to biology.
Lectures
Week 1
- L1. Introduction to amino acids
- L2. Introduction to proteins
- L3. Protein folding & misfolding
- L4. Introduction to proteomics
- L5. Lab session – Protein-protein interaction using label-free biosensors
Week 2
- L6. Sample preparation and pre-analytical factors
- L7. Sample preparation: Pre-analytical factors (contd.)
- L8. Sample preparation: Protein extraction and quantification
- L9. One-dimensional electrophoresis
- L10. Introduction to 2-DE
Week 3
- L11. 2-DE: Second dimension, staining & destaining
- L12. 2-DE: Gel analysis
- L13. 2-DE Applications
- L14. 2-DE Applications (contd.) & Challenges
- L15. Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR & data analysis
Week 4
- L16. 2D-DIGE: Basics
- L17. 2D-DIGE: Data analysis
- L18. 2D-DIGE: Applications
- L19. Systems biology and proteomics - I
- L20. Systems biology and proteomics - II
Week 5
- L21. Fundamentals of mass spectrometry
- L22. Chromatography technologies
- L23. Liquid chromatography
- L24. Mass spectrometry: Ionization sources
- L25. Mass spectrometry: Mass analyzers
Week 6
- L26. MALDI sample preparation and analysis
- L27. Hybrid mass spectrometry configurations
- L28. Lab session - Demonstration of Q-TOF MS technology
- L29. In-gel & in-solution digestion
- L30. Lab session - Sample preparation: tissue sample preservation technology
Week 7
- L31. Introduction to quantitative proteomics
- L32. SILAC: In vivo labeling
- L33. iTRAQ: In vitro labeling
- L34. TMT: In vitro labeling
- L35. Quantitative proteomics data analysis
Week 8
Syllabus and materials
At NPTEL, as the course publishes them.
Credits and licence
- Institution
- IIT Bombay
- Department
- Biotechnology
- Teacher
- Prof. Sanjeeva Srivastava
- Level
- Undergraduate
- Language
- English
- Subjects
- Biology
- Source
- NPTEL
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
- Who can watch
- NPTEL gives no rating. Invisible Network shows it to Tweens profiles and older, judged from what the source says about it. How courses are placed