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Learn · Introduction to Proteomics

Lecture 16 of 40Week 4

L16. 2D-DIGE: Basics

Prof. Sanjeeva Srivastava · IIT Bombay

Credits and licence

From
Introduction to Proteomics, IIT Bombay (at NPTEL)
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
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All the lectures

  1. L1. Introduction to amino acids YouTube
  2. L2. Introduction to proteins YouTube
  3. L3. Protein folding & misfolding YouTube
  4. L4. Introduction to proteomics YouTube
  5. L5. Lab session – Protein-protein interaction using label-free biosensors YouTube
  6. L6. Sample preparation and pre-analytical factors YouTube
  7. L7. Sample preparation: Pre-analytical factors (contd.) YouTube
  8. L8. Sample preparation: Protein extraction and quantification YouTube
  9. L9. One-dimensional electrophoresis YouTube
  10. L10. Introduction to 2-DE YouTube
  11. L11. 2-DE: Second dimension, staining & destaining YouTube
  12. L12. 2-DE: Gel analysis YouTube
  13. L13. 2-DE Applications YouTube
  14. L14. 2-DE Applications (contd.) & Challenges YouTube
  15. L15. Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR & data analysis YouTube
  16. L16. 2D-DIGE: Basics YouTube
  17. L17. 2D-DIGE: Data analysis YouTube
  18. L18. 2D-DIGE: Applications YouTube
  19. L19. Systems biology and proteomics - I YouTube
  20. L20. Systems biology and proteomics - II YouTube
  21. L21. Fundamentals of mass spectrometry YouTube
  22. L22. Chromatography technologies YouTube
  23. L23. Liquid chromatography YouTube
  24. L24. Mass spectrometry: Ionization sources YouTube
  25. L25. Mass spectrometry: Mass analyzers YouTube
  26. L26. MALDI sample preparation and analysis YouTube
  27. L27. Hybrid mass spectrometry configurations YouTube
  28. L28. Lab session - Demonstration of Q-TOF MS technology YouTube
  29. L29. In-gel & in-solution digestion YouTube
  30. L30. Lab session - Sample preparation: tissue sample preservation technology YouTube
  31. L31. Introduction to quantitative proteomics YouTube
  32. L32. SILAC: In vivo labeling YouTube
  33. L33. iTRAQ: In vitro labeling YouTube
  34. L34. TMT: In vitro labeling YouTube
  35. L35. Quantitative proteomics data analysis YouTube
  36. L36. Proteomics applications YouTube
  37. L37. Challenges in proteomics YouTube
  38. L38. OMICS and translational research YouTube
  39. L39. Lab session – Targeted proteomics using triple quadrupole mass spectrometry YouTube
  40. L40. Lab session – Targeted proteomics: multiple reaction monitoring YouTube