Course10210108271 lectures
Interactomics : Basics & Applications
Prof. Sanjeeva Srivastava · IIT Bombay
Lectures
Week 1
- Lecture 1 : Introduction to Proteomics
- Lecture 2 : Introduction to Interactomics
- Lecture 3 : High throughput platforms of interactomics: Protein arrays
- Lecture 4 : Cell-free expression based protein microarrays
- Lecture 5 : NAPPA:Recombinational Cloning, Basic workflow, Surface Chemistry, Printing and Assessment
Week 2
- Lecture 6 : NAPPA Technology and Protein Arrays-I
- Lecture 7 : NAPPA Technology and Protein Arrays-II
- Lecture 8 : Biomarkers: Harnessing the immune system for early detection of disease-I
- Lecture 9 : Biomarkers: Harnessing the immune system for early detection of disease-II
- Lecture 10 : Biomarkers: Harnessing the immune system for early detection of disease-III
Week 3
- Lecture 11 : NAPPA and its applications in study of antibody immune response in disease and in drug screening-I
- Lecture 12 : NAPPA and its applications in study of antibody immune response in disease and in drug screening-II
- Lecture 13 : NAPPA and its applications in study of antibody immune response in disease and in drug screening-III
- Lecture 14 : Using functional proteomics to identify biomarkers and therapeutic targets-I
- Lecture 15 : Using functional proteomics to identify biomarkers and therapeutic targets-II
Week 4
- Lecture 16 : Applications of protein microarrays in Malaria Research-I
- Lecture 17 : Applications of protein microarrays in Malaria Research-II
- Lecture 18 : Introduction to Bioprinting and Irisâ„¢ Optical QC Benefits-I
- Lecture 19 : Introduction to Bioprinting and Irisâ„¢ Optical QC Benefits-II
- Lecture 20 : Screening of autoantibody signatures in cancer patients: Lab demonstration
Week 5
- Lecture 21 : Basics of Image Scanning and data acquisition
- Lecture 22 : Applications of protein arrays in identification of autoantibody signatures-I
- Lecture 23 : Applications of protein arrays in identification of autoantibody signatures-II
- Lecture 24 : Applications of protein microarrays in deciphering PTMs and biological networks
- Lecture 25 : Basics and Applications of Reverse Phase Protein Arrays-I
Week 6
- Lecture 26 : Basics and Applications of Reverse Phase Protein Arrays-II
- Lecture 27 : Basics and Applications of Reverse Phase Protein Arrays-III
- Lecture 28 : An overview of label-free technologies
- Lecture 29 : Surface Plasmon Resonance- Principles and Assays-I
- Lecture 30 : Surface Plasmon Resonance- Principles and Assays-II
Week 7
- Lecture 31 : Basics of SPR: Surface chemistry
- Lecture 32 : Basics of SPR: Experimental design
- Lecture 33 : Protein immobilization for protein-protein interaction studies
- Lecture 34 : Protein-protein interaction study: Binding analysis
- Lecture 35 : Protein-protein interaction study: Kinetic analysis
Week 8
- Lecture 36 : Use of SPR in unravelling domain motif interactions of proteasomal assembly chaperones
- Lecture 37 : Protein-small molecule interaction study: Immobilization & binding analysis
- Lecture 38 : Protein-small molecule interaction study: Kinetic analysis
- Lecture 39 : An introduction to biolayer interferometry (BLI)and its applications in protein research
- Lecture 40 : Biomolecular interactions using Bio-Layer Interferometry (BLI)-I
Week 9
- Lecture 41 : Biomolecular interactions using Bio-Layer Interferometry (BLI)-II
- Lecture 42 : Lab session- An introduction to BioLayer Interferometry (BLI) and its applications in protein research
- Lecture 43 : Applications of label-free technologies-II
- Lecture 44 : Biomolecular interaction analytics using MicroScale Thermophoresis
- Lecture 45 : Mass Spectrometry coupled Interactomics-I
Week 10
- Lecture 46 : Mass Spectrometry coupled Interactomics-II
- Lecture 47 : Next-Generation Sequencing Technology- Ion Torrentâ„¢
- Lecture 48 : NGS Technology - Bioinformatics and data analysis-I
- Lecture 49 : NGS Technology - Bioinformatics and data analysis-II
- Lecture 50 : Next-Generation Sequencing Technology- Illumina
Week 11
- Lecture 51 : Agilent complete NGS target enrichment workflow for exomes, targeted panels and beyond
- Lecture 52 : The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome-I
- Lecture 53 : The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome-II
- Lecture 54 : Statistical Analysis-I
- Lecture 55 : Statistical Analysis-II
Week 12
- Lecture 56 : Secondary Data Analysis
- Lecture 57 : Pathway Enrichment and Network Analysis
- Lecture 58 : Data Repositories and Databases
- Lecture 59 : Application of multi-omics approach for better understanding of cancers
- Lecture 60 : Integrated Omics and Systems Biology- Conclusion
Live Session
Syllabus and materials
At NPTEL, as the course publishes them.
Credits and licence
- Institution
- IIT Bombay
- Department
- Biotechnology
- Teacher
- Prof. Sanjeeva Srivastava
- Level
- Undergraduate
- Language
- English
- Subjects
- Biology
- Source
- NPTEL
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
- Who can watch
- NPTEL gives no rating. Invisible Network shows it to Teens profiles and older, judged from what the source says about it. How courses are placed
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