Lecture 23 of 40Week 5
Lecture 23 : Conventional label based detection techniques for Protein microarrays
Prof. Sanjeeva Srivastava · IIT Bombay
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- Interactomics, IIT Bombay (at NPTEL)
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All the lectures
- Lecture 1 : Introduction to Interactomics
- Lecture 2 : An overview of label-free technologies
- Lecture 3 : An overview of surface plasmon resonance (SPR)
- Lecture 4 : An overview of surface plasmon resonance imaging (SPRi)
- Lecture 5 : Basics of SPR: Surface chemistry
- Lecture 6 : Basics of SPR: Experimental design
- Lecture 7 : Protein immobilization for protein-protein interaction studies
- Lecture 8 : Protein-protein interaction study: Binding analysis
- Lecture 9 : Protein-protein interaction study: Kinetic analysis
- Lecture 10 : Protein-small molecule interaction study: Immobilization & binding analysis
- Lecture 11 : Protein-small molecule interaction study: Kinetic analysis
- Lecture 12 : SPR: Interactive Session - I
- Lecture 13 : SPR: Interactive Session - II
- Lecture 14 : An overview of ellipsometry and interferometry techniques
- Lecture 15 : An introduction to BioLayer Interferometry (BLI) and its applications in protein research
- Lecture 16 : Kinetic analysis of protein-protein interaction using BLI
- Lecture 17 : Label-free quantification of proteins using BLI
- Lecture 18 : Diffraction-based biosensors - I
- Lecture 19 - Diffraction-based biosensors - II
- Lecture 20 - Nanotechniques in proteomics - I
- Lecture 21 - Nanotechniques in proteomics - II
- Lecture 22 : High throughputplatforms of interactomics: Protein arrays
- Lecture 23 : Conventional label based detection techniques for Protein microarrays
- Lecture 24 : Novel detection techniques for Protein microarrays
- Lecture 25 : Recombinational cloning and its application for Protein microarrays
- Lecture 26 : An introduction to Cell-free protein synthesis
- Lecture 27 : Cell-free synthesis based protein microarrays: PISA and NAPPA
- Lecture 28 : Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays
- Lecture 29 : Digging deeper into NAPPA: Basic Workflow
- Lecture 30 : Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment
- Lecture 31 : Application of cell free expression protein microarrays in biomarker discovery
- Lecture 32 : Application of cell-free expression protein microarrays in immunological studies
- Lecture 33 : Basics of microarray image scanning
- Lecture 34 : Software for Image scanning and data processing
- Lecture 35 : Microarray Data Analysis : Part I
- Lecture 36 : Microarray Data Analysis : Part II
- Lecture 37 : Application of protein microarray in biomarker discovery-I
- Lecture 38 : Application of protein microarray in biomarker discovery-II
- Lecture 39 : Systems biology and networks
- Lecture 40 : Challenges in proteomics