Learn · Mass spectrometry based proteomics
Lecture 12 of 28Week 2 : Basics of mass spectrometry
Lecture 9: Mass spectrometry: Mass analyzers
Prof. Sanjeeva Srivastava · IIT Bombay
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- Mass spectrometry based proteomics, IIT Bombay (at NPTEL)
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- CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
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- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
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All the lectures
- Introductory lecture
- Lecture 1: Introduction to proteomics
- Lecture 2: Proteomics and sample preparation
- Lecture 3: Bacterial protein extraction
- Lecture 4: In-gel digestion
- Lecture 5: Fundamentals of mass spectrometry
- Lab session 1.1 : Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR
- Lab Session 1.2: Demonstration of Q-TOF MS technology
- Lecture 6: Chromatography technologies
- Lecture 7: Liquid chromatography
- Lecture 8: Mass spectrometry: Ionization sources
- Lecture 9: Mass spectrometry: Mass analyzers
- Lecture 10: MALDI sample preparation and analysis
- Lab session 2.1: Global proteomics - experimental design and sample preparation
- Lab session 2.2: Global proteomics - instrumentation and data acquisition
- Lecture 11: Introduction to quantitative proteomics
- Lecture 12: Hybrid mass spectrometry configurations
- Lecture 13: SILAC: In Vivo labeling
- Lecture 14: iTRAQ: In Vitro labeling
- Lecture 15: TMT: In vitro labeling
- Lab session 3.1: Targeted proteomics - experimental design
- Lab session 3.2: Targeted proteomics - sample preparation, data acquisition and data analysis using skyline
- Lecture 16: Quantitative proteomics data analysis
- Lecture 17: Proteomics and Systems biology I
- Lecture 18: Proteomics & Systems biology II
- Lecture 19: Proteomics applications
- Lecture 20: Advances and challenges in proteomics
- Lab session 4.1: Protein-protein interaction using label-free biosensors