Learn · Mass spectrometry based proteomics

Lecture 13 of 28Week 2 : Basics of mass spectrometry

Lecture 10: MALDI sample preparation and analysis

Prof. Sanjeeva Srivastava · IIT Bombay

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Mass spectrometry based proteomics, IIT Bombay (at NPTEL)
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CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
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All the lectures

  1. Introductory lecture Video
  2. Lecture 1: Introduction to proteomics Video
  3. Lecture 2: Proteomics and sample preparation Video
  4. Lecture 3: Bacterial protein extraction Video
  5. Lecture 4: In-gel digestion Video
  6. Lecture 5: Fundamentals of mass spectrometry Video
  7. Lab session 1.1 : Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR Video
  8. Lab Session 1.2: Demonstration of Q-TOF MS technology Video
  9. Lecture 6: Chromatography technologies Video
  10. Lecture 7: Liquid chromatography Video
  11. Lecture 8: Mass spectrometry: Ionization sources Video
  12. Lecture 9: Mass spectrometry: Mass analyzers Video
  13. Lecture 10: MALDI sample preparation and analysis Video
  14. Lab session 2.1: Global proteomics - experimental design and sample preparation Video
  15. Lab session 2.2: Global proteomics - instrumentation and data acquisition Video
  16. Lecture 11: Introduction to quantitative proteomics Video
  17. Lecture 12: Hybrid mass spectrometry configurations Video
  18. Lecture 13: SILAC: In Vivo labeling Video
  19. Lecture 14: iTRAQ: In Vitro labeling Video
  20. Lecture 15: TMT: In vitro labeling Video
  21. Lab session 3.1: Targeted proteomics - experimental design Video
  22. Lab session 3.2: Targeted proteomics - sample preparation, data acquisition and data analysis using skyline Video
  23. Lecture 16: Quantitative proteomics data analysis Video
  24. Lecture 17: Proteomics and Systems biology I Video
  25. Lecture 18: Proteomics & Systems biology II Video
  26. Lecture 19: Proteomics applications Video
  27. Lecture 20: Advances and challenges in proteomics Video
  28. Lab session 4.1: Protein-protein interaction using label-free biosensors Video