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Course10210309750 lectures

Enzyme Sciences and Technology

Prof. Vishal Trivedi · IIT Guwahati

Start with lecture 1 At NPTEL

Lectures

Week 1: Introduction

  1. Lec 1: Introduction to Enzymes Video
  2. Lec 2: Basics of Enzyme Video
  3. Lec 3: Enzyme Classification (Part-I) Video
  4. Lec 4: Enzyme Classification (Part-II) Video
  5. Lec 5: Enzyme Nomenclature Video

Week 2: Enzyme Structure

  1. Lec 6: Primary Structure of Enzyme Video
  2. Lec 7: Determination of Primary Structure Video
  3. Lec 8: Secondary Structure of Protein Video
  4. Lec 9: Tertiary Structure of Enzyme(Part-I) Video
  5. Lec 10: Tertiary Structure of Enzyme(Part-II) Video
  6. Lec 11: Molecular Modelling of Enzyme Structure(Part-II) Video

Week 3: Enzyme Production (Part 1: Cloning)

  1. Lec 12: Identification of Enzyme Gene (Part-II) Video
  2. Lec 13: Identification of Enzyme Gene (Part-II) Video
  3. Lec 14: Polymerase Chain Reaction Video
  4. Lec 15: Enzymes in Molecular Cloning Video
  5. Lec 16: Cloning of Enzyme Coding Gene Video

Week 4: Enzyme Production (Part 2: Over-expression)

  1. Lec 17: DNA Delivery in host (Part-I) Video
  2. Lec 18: DNA Delivery in host (Part-II) Video
  3. Lec 19: Screening of Recombinant Clones Video
  4. Lec 20: Over-expression of Enzyme in host (Part-I) Video
  5. Lec 21: Over-expression of Enzyme in host (Part-II) Video
  6. Lec 22: Over-expression of Enzyme in host (Part-III) Video

Week 5: Enzyme Production (Part 3: Purification)

  1. Lec 23: Host Cell Disruption Methods Video
  2. Lec 24: Basics of Chromatography Video
  3. Lec 25: Chromatography (Part-I) Video
  4. Lec 26: Chromatography (Part-II) Video
  5. Lec 27: Chromatography (Part-III) Video

Week 6: Enzyme Catalyzed Biochemical Reactions

  1. Lec 28: Carbohydrate Metabolism Video
  2. Lec 29: Lipid Metabolism Video
  3. Lec 30: Amino acid Metabolism and Detoxification Video

Week 7: Enzyme Substrate Interactions

  1. "Lec 31: Enzyme-Substrate Interactions(Part-I Chromatographic Methods)" Video
  2. "Lec 32: Enzyme-Substrate Interactions(Part-II Spectroscopic Methods)" Video
  3. "Lec 33: Enzyme-Substrate Interactions(Part-III ITC)" Video
  4. "Lec 34: Enzyme-Substrate Interactions(Part-IV SPR)" Video

Week 8: Enzyme assay system and Kinetics

  1. "Lec 35: Enzyme Assay System(Part-I)" Video
  2. "Lec 36 Enzyme Assay System(Part-II)" Video
  3. "Lec 37: Enzyme Assay System(Part-III)" Video
  4. Lec 38: Enzyme Kinetics Video

Week 9: Enzyme Inhibitor Designing

  1. "Lec 39: Inhibitor Designing(Part-I: Traditional Approach)" Video
  2. "Lec 40: Inhibitor Designing(Part-II: Modern Approach)" Video
  3. "Lec 41: Inhibitor Designing(Part-III: Computational Approach)" Video

Week 10: Enzyme Inhibition Kinetics

  1. Lec 42: Enzyme Inhibition (Part-I) Video
  2. Lec 43: Enzyme Inhibition (Part-II) Video

Week 11: Enzyme Applications (Part-I)

  1. Lec 44: Application of Enzyme (Part-I Food Industry) Video
  2. Lec 45: Application of Enzyme (Part-II Medical Field) Video

Week 12: Enzyme Applications (Part-II)

  1. Lec 46: Enzyme in Drug Discovery Video
  2. Lec 47: Enzymes in Environmental Field Video

Live Session

  1. Live Session 13-02-2024 Video
  2. Live Session 20-03-2024 Video
  3. Live Session 09-04-2024 Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Guwahati
Department
Biotechnology
Teacher
Prof. Vishal Trivedi
Level
Undergraduate
Language
English
Subjects
Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
Who can watch
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