Course10210309349 lectures
Genome Editing And Engineering
Prof. Utpal Bora · IIT Guwahati
About
This course is designed for UG & PG students, research scholars and young scientists to introduce them to basics and applications of genome editing and engineering. The course starts with understanding of basic organization and structure of genome. It gives a brief overview on different DNA strand breaks and their repair mechanism. It introduces learners to theoretical basics of genetic engineering and discusses it’s limitations in tackling genetic problems of animals and plants. The key concepts of Genome editing are discussed thoroughly with emphasis on the major genome editing tools ZFN, TALEN and CRISP/Cas9. It discusses the biochemical basis of development of the genome editing tools, their design and their applications in various genetic conditions. It also discusses the scope and prospect of using these technologies in solving major genetic disease of human. The learners will also be acquainted with the ethical concerns associated with applications of genome editing and engineering in germline.
Intended audience: UG/PG/PhD/Scientist in industry
Prerequisites: 10+2 with Biology and Chemistry
Lectures
Week 1: Introduction to genetics and genetic engineering
- Lec 1: Introduction :Genes and Genome Organization
- Lec 2: History and Basics of Genetic Engineering
- Lec 3: Advantages and Limitations of Genetic Engineering
Week 2: Breakage and Repair of Genomic DNA
Week 3: Recombination
Week 4: Targeted genetic modification
Week 5: Zinc Finger Nuclease (ZFN) Technology
- Lec 1: Basics of Zinc Finger Nucleases
- Lec 2: Design of Zinc Finger Nucleases for genome editing
- Lec 3: Applications of Zinc Finger Nucleases - PART A
- Lec 4: Applications of Zinc Finger Nucleases - PART B
Week 6: Transcription activator - like effector nuclease (TALEN) Technology
- Lec 1: Basics of TALEN - PART A
- Lec 2: Basics of TALEN - PART B
- Lec 3: Design of TALEN for genome editing - PART A
- Lec 4: Design of TALEN for genome editing - PART B
- Lec 5: Application of TALEN - Part A
- Lec 6: Application of TALEN - Part B
Week 7: Clustered regularly interspaced short palindromic repeats (CRISPR) /Cas9 technology
- Lec 1: CRISPR system in bacteria - Part A
- Lec 2: CRISPR system in bacteria - Part B
- Lec 3: CRISPR/Cas9 in Genome Editing - Part A
- Lec 4: CRISPR/Cas9 in Genome Editing - Part B
- Lec 5: Applications of CRISPR/Cas9 Part A
- Lec 6: Applications of CRISPR/Cas9 Part B
- Lec 7: Computational Resources for CRISPR / Cas - Part A
- Lec 8: Computational Resources for CRISPR / Cas - Part B
Week 8:Applications of genome editing in treating human diseases
- Lec 1: Human cell engineering in diseases : Thalassemia - Part A
- Lec 2: Human cell engineering in diseases : Thalassemia - Part B
- Lec 3: Human cell engineering in diseases : Severe combined immunodeficiency (SCID) - Part A
- Lec 4: Human cell engineering in diseases : Severe combined immunodeficiency (SCID) - Part B
- Lec 5: Human cell engineering in diseases : Hemophilia - Part A
- Lec 6: Human cell engineering in diseases : Hemophilia - Part B
Week 9: Genome engineered Disease modelling
- Lec 1: Animal models - Part A
- Lec 2: Animal models - Part B
- Lec 3: iPSc models - Part A
- Lec 4: iPSc models - Part B
- Lec 5: Cancer disease models - Part A
- Lec 6: Cancer disease models - Part B
Week 10: Engineered immune cells for Cancer therapy
- Lec 1: Engineered immune cells for Cancer therapy (I) - Part A
- Lec 2: Engineered immune cells for Cancer therapy (I) - Part B
- Lec 3: Engineered immune cells for Cancer therapy (II) - Part A
- Lec 4: Engineered immune cells for Cancer therapy (II) - Part B
Week 11: Personalized Therapy
- Lec 1: History and Basics - Part A
- Lec 2: History and Basics - Part B
- Lec 3: Genome editing and personalized therapy
Week 12: Ethical concerns : Germ line gene editing
Syllabus and materials
At NPTEL, as the course publishes them.
Credits and licence
- Institution
- IIT Guwahati
- Department
- Biotechnology
- Teacher
- Prof. Utpal Bora
- Level
- Undergraduate
- Language
- English
- Subjects
- Biology
- Source
- NPTEL
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
- Who can watch
- NPTEL gives no rating. Invisible Network shows it to Teens profiles and older, judged from what the source says about it. How courses are placed