Course10210307441 lectures
Genetic Engineering: Theory And Application
Prof. Vishal Trivedi · IIT Guwahati
About
In the current MOOCs course I have put effort to briefly discuss about biotechnology, its scope and impact on human life with several customized products. The Development of technology and generation of product has multiple steps and understanding these steps are being covered in this course with a discussion of biotechnology application at the end. By the end of this course, student will be able to understand following aspects of biotechnology:
1. Basic metabolic pathways and their regulation.
2. Microbial growth kinetics with an emphasis on fermentation
3. Basic molecular biology tools used in biotechnology.
4. Basic methodology for product recovery and analysis.
INTENDED AUDIENCE : UG/PG/PhD/Scientist in industry
PREREQUISITES : General Biology 10+2
INDUSTRY SUPPORT : Biocon https://www.biocon.com/Jubilant Life Sciences www.jubl.com/ ,Shantha Biotechnics Ltd,Panacea Biotec,Other companies related to biotechnology
Lectures
Week 1: Introduction & Basics of Biological System
- Lec 1: Cellular Structure (Part I)
- Lec 2: Cellular Structure (Part II)
- Lec 3: Cellular Structure (Part III)
Week 2: Basics of Biological System
- Lec 1: Metabolic Reactions in Biological System
- Lec 2: Growth Media For Different Expression System
- Lec 3: Microbial Growth Kinetics
Week 3: Basics of Cloning (Part 1)
- Lec 1: Isolation of a Gene Fragment (Part I)
- Lec 2: Isolation of a Gene Fragment (Part II)
- Lec 3: Isolation of a Gene Fragment (Part III)
- Lec 4: Polymerase Chain Reaction
Week 4: Basics of Cloning (Part 2)
Week 5: Recombinant DNA Technology (Part 1)
- Lec 1: DNA Delivery In Host (Part I)
- Lec 2: DNA Delivery In Host (Part II)
- Lec 3: Screening of Recombinant Clones
Week 6: Recombinant DNA Technology (Part 2)
- Lec 1: Protein Production in Host (Part 1)
- Lec 2: Protein Production in Host (Part 2)
- Lec 3: Protein Production in Host (Part 3)
Week 7: Isolation and Purification of Product (Part 1)
- Lec 1: Product Recovery from Host Cells
- Lec 2: Basics of Chromatography (Part 1)
- Lec 3: Basics of Chromatography (Part 2)
- Lec 4: Ion-exchange Chromatography
- Lec 5: Hydrophobic Interaction chromatography
Week 8: Isolation and Purification of Product (Part 2)
- Lec 1: Gel Filtration chromatography (Part 1)
- Lec 2: Gel Filtration chromatography (Part 2)
- Lec 3: Affinity Chromatography (Part 1)
- Lec 4: Affinity Chromatography (Part 2)
- Lec 5: Affinity Chromatography (Part 3)
- Lec 6: Affinity Chromatography (Part 4)
Week 9: Characterization of Isolated Product (Part 1)
Week 10: Characterization of Isolated Product (Part 2)
Week 11
- Lec1: Biotechnology Applications (Part 1)
- Lec2: Biotechnology Applications (Part 2)
- Lec3: Biotechnology Applications (Part 3)
Week 12: Summary and conclusions
Syllabus and materials
At NPTEL, as the course publishes them.
Credits and licence
- Institution
- IIT Guwahati
- Department
- Biotechnology
- Teacher
- Prof. Vishal Trivedi
- Level
- Undergraduate
- Language
- English
- Subjects
- Biology
- Source
- NPTEL
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
- Who can watch
- NPTEL gives no rating. Invisible Network shows it to Tweens profiles and older, judged from what the source says about it. How courses are placed