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Experimental Biotechnology

Prof. Vishal Trivedi · IIT Guwahati

Start with lecture 1 At NPTEL

About

In the current MOOCs course I have put effort to briefly discuss different analytical techniques and their potential in solving the scientific problems. We are taking several scientific problems or questions which can be solved by using these techniques. By the end of this course, student will be able to understand: 1. Basics of Good Lab practices. 2. Understanding different analytical techniques and their applications. 3. Specific Scientific questions and their solutions. 4. Designing new experiments.

Intended audience: PG/PhD

Prerequisites: General Biology 10+2

Lectures

Week 1: Basics of Laboratory Research

  1. Lec 1: Good Lab Practices (Part 1) YouTube
  2. Lec 2: Good Lab Practices (Part 2) YouTube
  3. Lec 3: Operation of Laboratory Instruments (Part 1) YouTube
  4. Lec 4: Operation of Laboratory Instruments (Part 2) YouTube
  5. Lec 5: Operation of Laboratory Instruments (Part 3) YouTube
  6. Lec 6: Solution and Buffer Preparation YouTube

Week 2: Electrophoresis (Part 1)

  1. Lec 1: Basics of Electrophoresis (Part 1) YouTube
  2. Lec 2: Basics of Electrophoresis (Part 2) YouTube
  3. Lec 3: Horizontal Gel Electrophoresis YouTube

Week 3: Electrophoresis (Part 2)

  1. Lec 1: Different Varients of Gel Electrophoresis YouTube
  2. Lec 2: Scientific Questions (Part 1) YouTube
  3. Lec 3: Scientific Questions (Part 2) YouTube
  4. Lec 4: Scientific Questions (Part 3) YouTube
  5. Lec 5: Scientific Questions (Part 4) YouTube

Week 4: Chromatography (Part 1)

  1. Lec 1: Basics of chromatography (Part 1) YouTube
  2. Lec 2: Basics of chromatography (Part 2) YouTube
  3. Lec 3: Ion-exchange chromatography (Part 1) YouTube
  4. Lec 4: Ion-Exchange Chromatography (Part 2) YouTube
  5. Lec 5: Hydrophobic Interaction Chromatography YouTube

Week 5: Chromatography (Part 2)

  1. Lec 1: Gel filtration chromatography (Part 1) YouTube
  2. Lec 2: Gel Filtration Chromatography (Part 2) YouTube
  3. Lec 3: Gel Filtration Chromatography (Part 3) YouTube

Week 6: Chromatography (Part 3)

  1. Lec 1: Affinity chromatography (Part 1) YouTube
  2. Lec 2: Affinity Chromatography (Part 2) YouTube
  3. Lec 3: Affinity Chromatography (Part 3) YouTube
  4. Lec 4: Affinity Chromatography (Part 4) YouTube

Week 7: Immunological Techniques

  1. Lec 1: Antibody generation YouTube
  2. Lec 2: Antibody-Antigen Interaction (Part 1) YouTube
  3. Lec 3: Immunoassay YouTube
  4. Lec 4: Antibody-Antigen Interaction (Part 2) YouTube
  5. Lec 5: Antibody-Antigen Interaction (Part 3) YouTube

Week 8: Cell Biology (Part 1)

  1. Lec 1: Cell Culture Medium YouTube
  2. Lec 2: Cell Fractionation YouTube

Week 9: Cell Biology (Part 2)

  1. Lec 1: Microscopy (Part 1) YouTube
  2. Lec 2: Microscopy (Part 2) YouTube
  3. Lec 3: Cell Biology Experiments YouTube
  4. Lec 4: Flow cytometry YouTube

Week 10: Molecular Biology (Part 1)

  1. Lec 1: Polymerase chain reaction (Part 1) YouTube
  2. Lec 2: Polymerase chain reaction (Part 2) YouTube
  3. Lec 3: Polymerase chain reaction (Part 3) YouTube
  4. Lec 4: Polymerase chain reaction (Part 4) YouTube

Week 11: Molecular Biology (Part 2)

  1. Lec 1: Sequencing Techniques YouTube
  2. Lec 2: Blotting Techniques (Part-1) YouTube
  3. Lec 3: Blotting Techniques (Part-2) YouTube

Week 12: Summary and conclusions

  1. Lec 1: Designing Experiments YouTube

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Guwahati
Department
Biotechnology
Teacher
Prof. Vishal Trivedi
Level
Graduate
Language
English
Subjects
Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
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