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Course10210104925 lectures

Proteins and Gel-Based Proteomics

Prof. Sanjeeva Srivastava · IIT Bombay

Start with lecture 1 At NPTEL

About

This course introduces to the basic biology of proteins and gel-based proteomics. To obtain better understanding of cellular processes & regulation, there has been an increasing interest in studying proteomics. Proteomics techniques aim to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. These proteomic techniques typically aim to elucidate the expression, interaction, and cellular function of proteins.

The course will provide the basic understanding of amino acids and proteins. It would also provide detailed information on gel-based proteomic techniques such as SDS-PAGE and 2-DE. Some of the limitation of 2-DE has been overcome by advanced Difference gel electrophoresis (DIGE) technique, which will be discussed in this course. Protein identification using MALDI-TOF/TOF mass spectrometry will also be discussed. 2-DE in combination with mass spectrometry has become very powerful tool for proteomics applications and students can learn these tools by attending this course.

INTENDED AUDIENCE

Core course

All UG and PG students can attend this course.

It would be applied to B.Sc., M.Sc. and Ph.D

PRE-REQUISITES

B.Sc. Or M.Sc.

The target audiences of this course are required to have a basic biology and biochemistry background.

Lectures

Week 1

  1. L1. Introduction to amino acids YouTube
  2. L2. Introduction to proteins YouTube
  3. L3. Protein folding & misfolding YouTube
  4. L4. Protein purification techniques YouTube
  5. L5. Introduction to proteomics YouTube
  6. Lab Session-1.1: Demonstration of protein-protein interactions using label-free biosensors YouTube

Week 2

  1. L6. Systems biology and proteomics YouTube
  2. L7. Sample preparation and pre-analytical factors YouTube
  3. L8. Sample preparation: Pre-analytical factors (contd.) YouTube
  4. L9. Sample preparation: Protein extraction and quantification YouTube
  5. L10. One-dimensional electrophoresis YouTube
  6. Lab session-2.1: Sample preparation: Tissue sample preservation technology YouTube
  7. Lab session-2.2: Protein/peptide pre-fractionation using Off-gel fractionator YouTube

Week 3

  1. L11. 2-DE: Rehydration, IEF & Equilibration YouTube
  2. L12. 2-DE: Second dimension, staining & destaining YouTube
  3. L13. 2-DE: Gel analysis YouTube
  4. L14. 2-DE: Applications YouTube
  5. L15. 2-DE: Applications (contd.) & Challenges YouTube
  6. Lab session-3.1: Demonstration of gel analysis using IQTL software YouTube

Week 4

  1. L16. 2D-DIGE: Basics YouTube
  2. L17. 2D-DIGE: Data analysis YouTube
  3. L18. 2D-DIGE: Applications YouTube
  4. L19. Protein identification using MALDI-TOF/TOF YouTube
  5. L20. Proteomics experiment data analysis & challenges YouTube
  6. Lab session-4.1: Demonstration of Q-TOF MS technology YouTube

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Bombay
Department
Biotechnology
Teacher
Prof. Sanjeeva Srivastava
Level
Undergraduate
Language
English
Subjects
Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
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