Learn · Proteins and Gel-Based Proteomics
Lecture 22 of 25Week 4
L18. 2D-DIGE: Applications
Prof. Sanjeeva Srivastava · IIT Bombay
Credits and licence
- From
- Proteins and Gel-Based Proteomics, IIT Bombay (at NPTEL)
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
- Who can watch
- Tweens profiles and older. How courses are placed
All the lectures
- L1. Introduction to amino acids
- L2. Introduction to proteins
- L3. Protein folding & misfolding
- L4. Protein purification techniques
- L5. Introduction to proteomics
- Lab Session-1.1: Demonstration of protein-protein interactions using label-free biosensors
- L6. Systems biology and proteomics
- L7. Sample preparation and pre-analytical factors
- L8. Sample preparation: Pre-analytical factors (contd.)
- L9. Sample preparation: Protein extraction and quantification
- L10. One-dimensional electrophoresis
- Lab session-2.1: Sample preparation: Tissue sample preservation technology
- Lab session-2.2: Protein/peptide pre-fractionation using Off-gel fractionator
- L11. 2-DE: Rehydration, IEF & Equilibration
- L12. 2-DE: Second dimension, staining & destaining
- L13. 2-DE: Gel analysis
- L14. 2-DE: Applications
- L15. 2-DE: Applications (contd.) & Challenges
- Lab session-3.1: Demonstration of gel analysis using IQTL software
- L16. 2D-DIGE: Basics
- L17. 2D-DIGE: Data analysis
- L18. 2D-DIGE: Applications
- L19. Protein identification using MALDI-TOF/TOF
- L20. Proteomics experiment data analysis & challenges
- Lab session-4.1: Demonstration of Q-TOF MS technology