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Course10210682121 lectures

Animal Transgenesis: Principles and Methods

Prof. Himanshu Kumar · IISER Bhopal

Start with lecture 1 At NPTEL

Lectures

Week 1

  1. Common terms used in the course Video
  2. Why transgenesis is needed? Video
  3. Model organisms for transgenesis Video
  4. 3R Repalace Reduce Refine Video
  5. History of transgensis-I Video
  6. History of transgensis-II Video

Week 2

  1. General Principles of Transgenesis in Mouse Video
  2. History of Cell culture Video
  3. Cell culture Types & Basic Requirements Video
  4. Culture medium and Challenges with cell culture Video
  5. Methods for internalization of transgene, & Cell Preservation & Generation of transgene overexpressing mouse Video

Week 3

  1. Cell for transgenesis and Homologus recombination Video
  2. Targeting vector Video
  3. ES cells, Electroporation, Screening, & Founder or Chimera mice Video
  4. Germline transmission & Conditional knockout mice Video
  5. Example of conditional knockout mice & Temporal control of gene expression Video

Week 4

  1. Discovery, origin and Types of CRISPR-Cas9 System Video
  2. "CRISPR-Cas9 sgRNA and it’s modifications for gene editing" Video
  3. CRISPR-Cas9 version and it’s application Video
  4. "Genome-wide CRISPR-Cas9 screening and its variants application" Video
  5. CRISPR-Cas9 in therapeutics Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IISER Bhopal
Department
Biotechnology
Teacher
Prof. Himanshu Kumar
Level
Undergraduate
Language
English
Subjects
Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge. Coordinated by IIT Madras.
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