Learn · Animal Transgenesis: Principles and Methods
Lecture 16 of 21Week 3
Example of conditional knockout mice & Temporal control of gene expression
Prof. Himanshu Kumar · IISER Bhopal
Credits and licence
- From
- Animal Transgenesis: Principles and Methods, IISER Bhopal (at NPTEL)
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge. Coordinated by IIT Madras.
- Who can watch
- Tweens profiles and older. How courses are placed
All the lectures
- Common terms used in the course
- Why transgenesis is needed?
- Model organisms for transgenesis
- 3R Repalace Reduce Refine
- History of transgensis-I
- History of transgensis-II
- General Principles of Transgenesis in Mouse
- History of Cell culture
- Cell culture Types & Basic Requirements
- Culture medium and Challenges with cell culture
- Methods for internalization of transgene, & Cell Preservation & Generation of transgene overexpressing mouse
- Cell for transgenesis and Homologus recombination
- Targeting vector
- ES cells, Electroporation, Screening, & Founder or Chimera mice
- Germline transmission & Conditional knockout mice
- Example of conditional knockout mice & Temporal control of gene expression
- Discovery, origin and Types of CRISPR-Cas9 System
- "CRISPR-Cas9 sgRNA and it’s modifications for gene editing"
- CRISPR-Cas9 version and it’s application
- "Genome-wide CRISPR-Cas9 screening and its variants application"
- CRISPR-Cas9 in therapeutics