Learn · Animal Transgenesis: Principles and Methods

Lecture 5 of 21Week 1

History of transgensis-I

Prof. Himanshu Kumar · IISER Bhopal

Credits and licence

From
Animal Transgenesis: Principles and Methods, IISER Bhopal (at NPTEL)
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge. Coordinated by IIT Madras.
Who can watch
Tweens profiles and older. How courses are placed

All the lectures

  1. Common terms used in the course Video
  2. Why transgenesis is needed? Video
  3. Model organisms for transgenesis Video
  4. 3R Repalace Reduce Refine Video
  5. History of transgensis-I Video
  6. History of transgensis-II Video
  7. General Principles of Transgenesis in Mouse Video
  8. History of Cell culture Video
  9. Cell culture Types & Basic Requirements Video
  10. Culture medium and Challenges with cell culture Video
  11. Methods for internalization of transgene, & Cell Preservation & Generation of transgene overexpressing mouse Video
  12. Cell for transgenesis and Homologus recombination Video
  13. Targeting vector Video
  14. ES cells, Electroporation, Screening, & Founder or Chimera mice Video
  15. Germline transmission & Conditional knockout mice Video
  16. Example of conditional knockout mice & Temporal control of gene expression Video
  17. Discovery, origin and Types of CRISPR-Cas9 System Video
  18. "CRISPR-Cas9 sgRNA and it’s modifications for gene editing" Video
  19. CRISPR-Cas9 version and it’s application Video
  20. "Genome-wide CRISPR-Cas9 screening and its variants application" Video
  21. CRISPR-Cas9 in therapeutics Video