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Course10410809742 lectures

Multidimensional NMR Spectroscopy for Structural Studies of Biomolecules

Prof. Hanudatta S. Atreya · IISc Bangalore

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Lectures

Introduction to Basics of NMR

  1. Introduction to NMR spectroscopy Video
  2. Energy levels in NMR spectroscopy: Quantum mechanical model and Vector model Video
  3. Observing the NMR signal Video
  4. Basic concepts in 1D NMR: Chemical shift and Spin-spin coupling Video
  5. Basic concepts in 1D NMR: Nuclear Spin Relaxation, 1H NMR and 13C NMR Video

Two dimensional NMR

  1. Basic concepts in 2D NMR spectroscopy Video
  2. Principles of 2D correlation spectroscopy COSY Video
  3. Principles of 2D Total correlation spectroscopy (TOCSY) Video
  4. 2D Nuclear Overhauser Effect Spectroscopy (NOESY) Video
  5. 2D NOESY and 2D ROESY Video

Important 2D NMR experiments for Biomolecules

  1. Principles of 2D Heteronuclear NMR Video
  2. 2D Heteronuclear NMR: HSQC Video
  3. Heteronuclear multiple quantum coherence (HMQC) and single quantum coherence (HSQC) - Part I Video
  4. Heteronuclear multiple quantum coherence (HMQC) and single quantum coherence (HSQC) - Part II Video
  5. 2D HSQC-TOCSY Video

3D NMR Spectroscopy

  1. 3D NMR Spectroscopy - part I Video
  2. 3D NMR Spectroscopy - part II Video
  3. 3D HNCA and 3D HNCO Video
  4. 3D HNCACB and 3D HN(CO)CACB Video
  5. Protein Backbone resonance assignment and side chain resonance assignment Video

Basics of Protein Structure

  1. Basic concepts of protein structure Video
  2. Introduction to Structure Determination of Bio-Molecules by NMR Video
  3. Over-expression of proteins in Bacteria Video
  4. Isotope labeling of proteins for NMR studies - Part I Video
  5. Isotope labeling of proteins for NMR studies - Part II Video

Isotope Labelling

  1. Isotope labeling of proteins for NMR studies - Part III Video
  2. Isotope labeling of proteins for NMR studies - Part IV Video
  3. Resonance assignments of Proteins - Part I Video
  4. Resonance assignments of Proteins - Part II Video
  5. Resonance assignments of Proteins - Part III Video

Resonance assignment of Proteins with NMR and Structure Determination

  1. Determination of protein secondary structure from NMR data: CSI method Video
  2. Determination of protein secondary structure from NMR data: J coupling based method Video
  3. Determination of protein tertiary structure from NMR data - part I Video
  4. 3D NOESY HSQC Video
  5. Determination of protein tertiary structure from NMR data - part II Video

NMR Experiments for Studying Protein-Ligand Interactions

  1. Understanding Protein ligand interaction by NMR: Chemical shift perturbation Video
  2. Understanding Protein ligand interaction by NMR: Chemical exchange Video
  3. Understanding Protein ligand interaction by NMR: T2 Filter Video
  4. Understanding Protein ligand interaction by NMR: STD NMR Video
  5. Understanding Protein ligand interaction by NMR: Transfer NOE NMR Video
  6. Understanding Protein ligand interaction by NMR : Diffusion ordered Spectroscopy (DOSY) - Part I Video
  7. Understanding Protein ligand interaction by NMR : Diffusion ordered Spectroscopy (DOSY) - Part II Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IISc Bangalore
Department
Chemistry and Biochemistry
Teacher
Prof. Hanudatta S. Atreya
Level
Undergraduate
Language
English
Subjects
Chemistry
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
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