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Course10410805640 lectures

Eukaryotic Gene Expression - basics and benefits

Prof. P.N. Rangarajan · IISc Bangalore

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Lectures

CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORS

  1. Eukaryotic RNA polymerases and basal transcription factors Video
  2. Diversity in core promoter elements Video
  3. Diversity in general transcription factors Video
  4. Proximal & Distal Promoter Elements, Enhancers and Silencers, Gene-specific Regulators Video

DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORS

  1. Transcription factors - DNA binding domains Video
  2. Transcription factors - transcription activation domain Video

ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATION

  1. Role of chromatin in eukaryotic gene regulation Video
  2. Role of histones in eukaryotic gene regulation Video
  3. Role of DNA methylation in eukaryotic gene regulation Video
  4. Chromatin remodelling & gene regulation Video

SYNTHESIS OF mRNA, rRNA & tRNA

  1. mRNA processing - Role of RNA Pol II in mRNA capping and mRNA splicing Video
  2. mRNA processing - Role of RNA Pol II in polyadenylation & mRNA editing Video
  3. Regulation of RNA Pol I transcription Video
  4. Regulation of RNA Pol III transcription Video

REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORS

  1. Signal Transduction Pathways - Introduction Video
  2. Regulation of gene expression by cyclicAMP Video
  3. Regulation of gene expression by second messengers other than cAMP Video
  4. Regulation of gene expression by Protein Kinase C Video
  5. Regulation of gene expression by Growth factors Video
  6. Regulation of gene expression by cytokines Video

REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORS

  1. Regulation of gene expression by steroid hormones Video
  2. Regulation of gene expression by type II nuclear receptors Video
  3. Mechanism of transcriptional activation by nuclear receptors Video

REGULATION OF GENE EXPRESSION DURING DEVELOPMENT

  1. Gene Regulation during Drosophila Development Video
  2. Signal transduction pathways involved in embryonic development Video
  3. Homeotic genes Video
  4. Epigenetic regulation of gene expression during development Video
  5. Embryonic stem cells and Transcription factor-mediated epigenetic reprogramming Video

RECOMBINANT PROTEIN EXPRESSION SYSTEMS

  1. Cloning and Expression vectors Video
  2. Eukaryotic protein expression systems - I Video
  3. Eukaryotic protein expression systems - II Video
  4. Eukaryotic protein expression systems - III: Gene expression in mammalian cells using viral vectors Video

GENE THERAPY AND TRANSGENIC TECHNOLOGY

  1. Human Gene Therapy Video
  2. DNA vaccines Video
  3. Transgenic animals Video
  4. Transgenic plants Video
  5. Knockout mice Video

RECENT ADVANCES IN EUKARYOTIC GENE EXPRESSION

  1. Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi) Video
  2. Genomics & Proteomics Video
  3. Metabolic Engineering & Synthetic Biology Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IISc Bangalore
Department
Chemistry and Biochemistry
Teacher
Prof. P.N. Rangarajan
Level
Undergraduate
Language
English
Subjects
Chemistry
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
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