Learn · Multidimensional NMR Spectroscopy for Structural Studies of Biomolecules

Lecture 38 of 42NMR Experiments for Studying Protein-Ligand Interactions

Understanding Protein ligand interaction by NMR: T2 Filter

Prof. Hanudatta S. Atreya · IISc Bangalore

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Multidimensional NMR Spectroscopy for Structural Studies of Biomolecules, IISc Bangalore (at NPTEL)
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All the lectures

  1. Introduction to NMR spectroscopy Video
  2. Energy levels in NMR spectroscopy: Quantum mechanical model and Vector model Video
  3. Observing the NMR signal Video
  4. Basic concepts in 1D NMR: Chemical shift and Spin-spin coupling Video
  5. Basic concepts in 1D NMR: Nuclear Spin Relaxation, 1H NMR and 13C NMR Video
  6. Basic concepts in 2D NMR spectroscopy Video
  7. Principles of 2D correlation spectroscopy COSY Video
  8. Principles of 2D Total correlation spectroscopy (TOCSY) Video
  9. 2D Nuclear Overhauser Effect Spectroscopy (NOESY) Video
  10. 2D NOESY and 2D ROESY Video
  11. Principles of 2D Heteronuclear NMR Video
  12. 2D Heteronuclear NMR: HSQC Video
  13. Heteronuclear multiple quantum coherence (HMQC) and single quantum coherence (HSQC) - Part I Video
  14. Heteronuclear multiple quantum coherence (HMQC) and single quantum coherence (HSQC) - Part II Video
  15. 2D HSQC-TOCSY Video
  16. 3D NMR Spectroscopy - part I Video
  17. 3D NMR Spectroscopy - part II Video
  18. 3D HNCA and 3D HNCO Video
  19. 3D HNCACB and 3D HN(CO)CACB Video
  20. Protein Backbone resonance assignment and side chain resonance assignment Video
  21. Basic concepts of protein structure Video
  22. Introduction to Structure Determination of Bio-Molecules by NMR Video
  23. Over-expression of proteins in Bacteria Video
  24. Isotope labeling of proteins for NMR studies - Part I Video
  25. Isotope labeling of proteins for NMR studies - Part II Video
  26. Isotope labeling of proteins for NMR studies - Part III Video
  27. Isotope labeling of proteins for NMR studies - Part IV Video
  28. Resonance assignments of Proteins - Part I Video
  29. Resonance assignments of Proteins - Part II Video
  30. Resonance assignments of Proteins - Part III Video
  31. Determination of protein secondary structure from NMR data: CSI method Video
  32. Determination of protein secondary structure from NMR data: J coupling based method Video
  33. Determination of protein tertiary structure from NMR data - part I Video
  34. 3D NOESY HSQC Video
  35. Determination of protein tertiary structure from NMR data - part II Video
  36. Understanding Protein ligand interaction by NMR: Chemical shift perturbation Video
  37. Understanding Protein ligand interaction by NMR: Chemical exchange Video
  38. Understanding Protein ligand interaction by NMR: T2 Filter Video
  39. Understanding Protein ligand interaction by NMR: STD NMR Video
  40. Understanding Protein ligand interaction by NMR: Transfer NOE NMR Video
  41. Understanding Protein ligand interaction by NMR : Diffusion ordered Spectroscopy (DOSY) - Part I Video
  42. Understanding Protein ligand interaction by NMR : Diffusion ordered Spectroscopy (DOSY) - Part II Video