Learn · Multidimensional NMR Spectroscopy for Structural Studies of Biomolecules
Lecture 25 of 42Basics of Protein Structure
Isotope labeling of proteins for NMR studies - Part II
Prof. Hanudatta S. Atreya · IISc Bangalore
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- Multidimensional NMR Spectroscopy for Structural Studies of Biomolecules, IISc Bangalore (at NPTEL)
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All the lectures
- Introduction to NMR spectroscopy
- Energy levels in NMR spectroscopy: Quantum mechanical model and Vector model
- Observing the NMR signal
- Basic concepts in 1D NMR: Chemical shift and Spin-spin coupling
- Basic concepts in 1D NMR: Nuclear Spin Relaxation, 1H NMR and 13C NMR
- Basic concepts in 2D NMR spectroscopy
- Principles of 2D correlation spectroscopy COSY
- Principles of 2D Total correlation spectroscopy (TOCSY)
- 2D Nuclear Overhauser Effect Spectroscopy (NOESY)
- 2D NOESY and 2D ROESY
- Principles of 2D Heteronuclear NMR
- 2D Heteronuclear NMR: HSQC
- Heteronuclear multiple quantum coherence (HMQC) and single quantum coherence (HSQC) - Part I
- Heteronuclear multiple quantum coherence (HMQC) and single quantum coherence (HSQC) - Part II
- 2D HSQC-TOCSY
- 3D NMR Spectroscopy - part I
- 3D NMR Spectroscopy - part II
- 3D HNCA and 3D HNCO
- 3D HNCACB and 3D HN(CO)CACB
- Protein Backbone resonance assignment and side chain resonance assignment
- Basic concepts of protein structure
- Introduction to Structure Determination of Bio-Molecules by NMR
- Over-expression of proteins in Bacteria
- Isotope labeling of proteins for NMR studies - Part I
- Isotope labeling of proteins for NMR studies - Part II
- Isotope labeling of proteins for NMR studies - Part III
- Isotope labeling of proteins for NMR studies - Part IV
- Resonance assignments of Proteins - Part I
- Resonance assignments of Proteins - Part II
- Resonance assignments of Proteins - Part III
- Determination of protein secondary structure from NMR data: CSI method
- Determination of protein secondary structure from NMR data: J coupling based method
- Determination of protein tertiary structure from NMR data - part I
- 3D NOESY HSQC
- Determination of protein tertiary structure from NMR data - part II
- Understanding Protein ligand interaction by NMR: Chemical shift perturbation
- Understanding Protein ligand interaction by NMR: Chemical exchange
- Understanding Protein ligand interaction by NMR: T2 Filter
- Understanding Protein ligand interaction by NMR: STD NMR
- Understanding Protein ligand interaction by NMR: Transfer NOE NMR
- Understanding Protein ligand interaction by NMR : Diffusion ordered Spectroscopy (DOSY) - Part I
- Understanding Protein ligand interaction by NMR : Diffusion ordered Spectroscopy (DOSY) - Part II