Mass Spectrometry and its application in Molecular Medicine

Lectures

Week 1

1. Introduction to soft ionisation mass spectrometry: ESI-MS and MALDI-MS Video
2. Isotopic distribution of signals in mass spectra and deconvolution of mass spectra Video
3. Resolution of mass spectra, accuracy in mass measurement and introduction of molecular medicine Video
4. Mass spectra of human hemoglobin and glycated hemoglobin, a diagnostic marker of diabetes mellitus and glutathionyl hemoglobin, a marker of oxidative stress Video
5. Mass spectrometry based quantification of oxidative stress in patients with chronic renal failure Video

Week 2

6. Introduction to tandem mass spectrometry and principle of de novo sequencing Video
7. Chemical modification and enzyme digestion of a peptide: A step towards de novo sequencing Video
8. De novo sequencing of a peptide from chemical derivatization and tandem mass spectra Video
9. De novo sequencing: Identification of isobaric amino acid and disulfide connectivity Video
10. De novo sequencing of a tryptic peptide from tandem mass spectra Video

Week 3

11. De novo sequencing from tandem-MS and SPITC derivatization to simplify tandem-MS Video
12. Routine diagnosis of Hemoglobinopathy: A disease caused by genetic variant of hemoglobin Video
13. Hemoglobinopathies: Identification and characterisation using bottom up proteomics approach--Part-1 Video
14. Hemoglobinopathies: Identification and characterisation using bottom up proteomics approach--Part-2 Video
15. A genetic variant of hemoglobin causing false elevation of HbA1c and hence false diagnosis of diabetes mellitus Video

Week 4

16. Nano-LC-ESI-MS based analysis of a proteome - A bottom up proteomics approach Video
17. Iron salts as food fortificants to combat Iron Deficiency Anemia Video
18. Nano ferric pyrophosphate as a food fortificant in a rat model: Bioavailability and toxicity assessment Video
19. Nano ferric pyrophosphate as a food fortificant in a rat model: Toxicity assessment using biochemical, histopathological and proteomics approach Video
20. Nano ferric pyrophosphate as a food fortificant in a rat model: Exploring biomarker of nano particle induced toxicity in rat plasma Video

Week 5

21. Biomarker of nano particles induced toxicity in a rat model: NanoLC-ESI-MS based plasma proteomics approach Video
22. Quantitative proteomics of rat plasma proteins: Absolute quantification of Fetuin B, a biomarker of nano particle toxicity Video
23. 2D gel electrophoresis based proteomics: principles and methods Video
24. Differential plasma proteomics: 2D gel electrophoresis based approach Video
25. Analysis of 2D gel spots followed by in-gel digestion to identify proteins usng MALDI-MS platform Video

Week 6

26. Mass spectrometry based absolute quantification of human plasma proteins Video
27. Absolute quantification of plasma Apolipoprotein-AIV: A probale biomarker of attempted suicide Video
28. MALDI-MS based identification of microorganism in clinical samples Video
29. Native mass spectrometry using nano-ESI-MS platform Video
30. Analysis of an array of glycated hemoglobin in patients with poorly controlled diabetes mellitus Video

Week 7

31. Molecular mechanism of sickle hemoglobin polymerisation Video
32. Inhibition of sickle hemoglobin polymerisation upon glutathionylation Video
33. Structural analysis of desorbed hemoglobin from nano particle surface Video
34. Functional assay of human hemoglobin and its variants Video
35. Application of native mas spectrometry to calculate equilibrium constant Video

Week 8

36. Bioconjugation of para-benzoquinone of cigarette smoke with human hemoglobin Video
37. Bioconjugation of human hemoglobin with quinone as airborne enviroment pollutants Video
38. Ion mobility mass spectrometry Video
39. Introduction to hydrogen-deuterium-exchange mass spectrometry Video
40. Rate law of hydrogen-deuterium-exchange kinetics Video

Week 9

41. Molecular insights in inhibition of HbS polymerisation upon oxygenation monitored by HDX-MS platform-part-I Video
42. Molecular insights in inhibition of HbS polymerisation upon oxygenation monitored by HDX-MS platform-part-II Video
43. Molecular insights in inhibition of HbS polymerisation upon oxygenation -part-III- and upon glutathionylation-part-I-monitored by HDX-MS platform Video
44. Molecular insights in inhibition of HbS polymerisation upon glutathionylation-part-II Video
45. N-acetyl cysteine as an antisickling agent in sickle cell disease Video

Week 10

46. Effect of quinones on morphology of human RBC Video
47. Effect-of-NAC-to neturalise-quinone-and-in-vivo-HDX with human RBCs as a model system Video
48. Application of HDX-MS in structural analysis of human hemoglobin in vivo Video
49. Application of HDX-MS in structural analysis of hemoglobin in vivo-and-desorbed-hemoglobin from nano particle-surface Video
50. Application-of-HDX-MS-and -CD-spectroscopy-to-monitor-structural changes-of human hemoglobin from nano particle surface Video

Week 11

51. Structure-function correlation of Hb Beckman, a genetic variant of human hemoglobin, using HDX-MS Video
52. Structure-function correlation of glutathionyl hemoglobin, a posttranslational variant of human hemoglobin, using HDX-MS Video
53. Imaging mass spectromery of biological tissue samples using MALDI mass spectrometry platform Video
54. Kidney tissue imaging in a nano particle exposed rat model Video
55. Imaging of kidney tissues of NP exposed rats and imaging of human prostate tissue Video

Week 12

56. Imaging of human prostate cancer tissue using MALDI-MS Video
57. Imaging of human prostate cancer tissue using MALDI-MS and analysis of tissue proteomics data Video
58. Desorption Electrospary Ionisation based Mass Spectrometric Imaging of Prostate Cancer Tissue Video
59. Fast photochemical oxidation of protein combined to mass spectrometry for prtein footprinting both in vitro and in vivo Video
60. Biosimilar-monitored through mass spectrometric techniques Video