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An illustration of a cross-section of a small portion of an E. coli cell, showing the cell wall, the cytoplasmic area packed with ribosomes, tRNA, mRNA, enzymes, and the nucleus filled with DNA.

Course5.08J / 7.08J,7.8049 lectures

Biological Chemistry II

Prof. Joanne Stubbe and Prof. Elizabeth Nolan · Massachusetts Institute of Technology

Start with lecture 1 At MIT OpenCourseWare

About

This course is an advanced treatment of biochemical mechanisms that underlie biological processes. Topics include macromolecular machines such as the ribosome, the proteasome, fatty acid synthases as a paradigm for polyketide synthases and non-ribosomal polypeptide synthases, and polymerases. Emphasis will be given to the experimental methods used to unravel how these processes fit into the cellular context as well as the coordinated regulation of these processes.

Lectures

Lecture & Recitation Videos

  1. Lecture 1: Introduction 36 min · Video
  2. Lecture 2: Protein Synthesis 1 51 min · Video
  3. Recitation 1: Determining, Analyzing, and Understanding Protein Structures 30 min · Video
  4. Lecture 3: Protein Synthesis 2 51 min · Video
  5. Lecture 4: Protein Synthesis 3 52 min · Video
  6. Lecture 5: Protein Synthesis 4 52 min · Video
  7. Recitation 2: Pre-Steady State and Steady-State Kinetic Methods Applied to Translation 54 min · Video
  8. Lecture 6: Protein Synthesis 5 53 min · Video
  9. Lecture 7: Protein Synthesis 6 51 min · Video
  10. Lecture 8: Protein Folding 1 52 min · Video
  11. Recitation 3: Pre-Steady State and Steady-State Kinetic Methods Applied to Translation 56 min · Video
  12. Lecture 9: Protein Folding 2 53 min · Video
  13. Lecture 10: Protein Folding 3 47 min · Video
  14. Lecture 11: Protein Folding 4 52 min · Video
  15. Recitation 4: Purification of Native and Mutant Ribosomes, Protein Purification 1 h · Video
  16. Lecture 12: Protein Degradation 1 52 min · Video
  17. Lecture 13: Protein Degradation 2 52 min · Video
  18. Lecture 14: Protein Degradation 3 49 min · Video
  19. Recitation 5: Overview of Cross-Linking, Including Photo-Reactive Cross-Linking Methods 51 min · Video
  20. Lecture 15: PK and NRP Synthases 1 50 min · Video
  21. Lecture 16: PK and NRP Synthases 2 56 min · Video
  22. Recitation 6: Macromolecular Electron Microscopy Applied to Fatty Acid Synthase 1 h 1 min · Video
  23. Lecture 17: PK and NRP Synthases 3 52 min · Video
  24. Lecture 18: PK and NRP Synthases 4 55 min · Video
  25. Recitation 7: Application of Single Molecule Methods to Understand Macromolecular Machines And Protein Degradation 54 min · Video
  26. 19. Cholesterol Biosynthesis 1 Video
  27. Lecture 20: Cholesterol Biosynthesis 2 52 min · Video
  28. Lecture 21: Cholesterol Biosynthesis 3 & Cholesterol Homeostasis 1 54 min · Video
  29. Recitation 8: Application of CRISPR to Study Cholesterol Regulation 55 min · Video
  30. Lecture 22: Cholesterol Homeostasis 2 50 min · Video
  31. Lecture 23: Cholesterol Homeostasis 3 54 min · Video
  32. Lecture 24: Cholesterol Homeostasis 4 50 min · Video
  33. Recitation 9: Cholesterol Homeostasis and Sensing 55 min · Video
  34. Lecture 25: Cholesterol Homeostasis 5 & Metal Ion Homeostasis 1 54 min · Video
  35. Lecture 26: Metal Ion Homeostasis 2 52 min · Video
  36. Lecture 27: Metal Ion Homeostasis 3 53 min · Video
  37. Recitation 10: Metal-Binding Studies and Dissociation Constant Determination 1 h · Video
  38. Lecture 28: Metal Ion Homeostasis 4 52 min · Video
  39. Lecture 29: Metal Ion Homeostasis 5 53 min · Video
  40. Recitation 11: Mass Spectrometry 55 min · Video
  41. Lecture 30: Metal Ion Homeostasis 6 53 min · Video
  42. Lecture 31: Metal Ion Homeostasis 7 & Reactive Oxygen Species 1 53 min · Video
  43. Recitation 12: Mass Spectrometry of the Cysteine Proteome 54 min · Video
  44. Lecture 32: Reactive Oxygen Species 2 50 min · Video
  45. Lecture 33: Reactive Oxygen Species 3 55 min · Video
  46. Lecture 34: Reactive Oxygen Species 4 & Nucleotide Metabolism 1 50 min · Video
  47. Recitation 13: Fluorescence Methods 54 min · Video
  48. Lecture 35: Nucleotide Metabolism 2 49 min · Video
  49. Lecture 36: Nucleotide Metabolism 3 51 min · Video

Syllabus and materials

At MIT OpenCourseWare, as the course publishes them.

Credits and licence

Institution
Massachusetts Institute of Technology
Department
Chemistry; Biology
Taught by
Prof. Joanne Stubbe, Prof. Elizabeth Nolan
Term
Spring 2016
Level
Undergraduate
Language
English
Subjects
Chemistry, Biology
Source
MIT OpenCourseWare
Licence
CC BY-NC-SA 4.0, as MIT OpenCourseWare states it. Non-commercial use only. MIT OpenCourseWare's terms
Attribution
Prof. Joanne Stubbe, Prof. Elizabeth Nolan. Biological Chemistry II. Spring 2016. Massachusetts Institute of Technology: MIT OpenCourseWare, https://ocw.mit.edu/. License: Creative Commons BY-NC-SA.
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