Learn · Mass Spectrometry and its application in Molecular Medicine
Lecture 2 of 60Week 1
Isotopic distribution of signals in mass spectra and deconvolution of mass spectra
Prof. Amit Kumar Mandal · IISER Kolkata
Credits and licence
- From
- Mass Spectrometry and its application in Molecular Medicine, IISER Kolkata (at NPTEL)
- Licence
- CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
- Attribution
- NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge. Coordinated by IIT Madras.
- Who can watch
- Teens profiles and older. How courses are placed
All the lectures
- Introduction to soft ionisation mass spectrometry: ESI-MS and MALDI-MS
- Isotopic distribution of signals in mass spectra and deconvolution of mass spectra
- Resolution of mass spectra, accuracy in mass measurement and introduction of molecular medicine
- Mass spectra of human hemoglobin and glycated hemoglobin, a diagnostic marker of diabetes mellitus and glutathionyl hemoglobin, a marker of oxidative stress
- Mass spectrometry based quantification of oxidative stress in patients with chronic renal failure
- Introduction to tandem mass spectrometry and principle of de novo sequencing
- Chemical modification and enzyme digestion of a peptide: A step towards de novo sequencing
- De novo sequencing of a peptide from chemical derivatization and tandem mass spectra
- De novo sequencing: Identification of isobaric amino acid and disulfide connectivity
- De novo sequencing of a tryptic peptide from tandem mass spectra
- De novo sequencing from tandem-MS and SPITC derivatization to simplify tandem-MS
- Routine diagnosis of Hemoglobinopathy: A disease caused by genetic variant of hemoglobin
- Hemoglobinopathies: Identification and characterisation using bottom up proteomics approach--Part-1
- Hemoglobinopathies: Identification and characterisation using bottom up proteomics approach--Part-2
- A genetic variant of hemoglobin causing false elevation of HbA1c and hence false diagnosis of diabetes mellitus
- Nano-LC-ESI-MS based analysis of a proteome - A bottom up proteomics approach
- Iron salts as food fortificants to combat Iron Deficiency Anemia
- Nano ferric pyrophosphate as a food fortificant in a rat model: Bioavailability and toxicity assessment
- Nano ferric pyrophosphate as a food fortificant in a rat model: Toxicity assessment using biochemical, histopathological and proteomics approach
- Nano ferric pyrophosphate as a food fortificant in a rat model: Exploring biomarker of nano particle induced toxicity in rat plasma
- Biomarker of nano particles induced toxicity in a rat model: NanoLC-ESI-MS based plasma proteomics approach
- Quantitative proteomics of rat plasma proteins: Absolute quantification of Fetuin B, a biomarker of nano particle toxicity
- 2D gel electrophoresis based proteomics: principles and methods
- Differential plasma proteomics: 2D gel electrophoresis based approach
- Analysis of 2D gel spots followed by in-gel digestion to identify proteins usng MALDI-MS platform
- Mass spectrometry based absolute quantification of human plasma proteins
- Absolute quantification of plasma Apolipoprotein-AIV: A probale biomarker of attempted suicide
- MALDI-MS based identification of microorganism in clinical samples
- Native mass spectrometry using nano-ESI-MS platform
- Analysis of an array of glycated hemoglobin in patients with poorly controlled diabetes mellitus
- Molecular mechanism of sickle hemoglobin polymerisation
- Inhibition of sickle hemoglobin polymerisation upon glutathionylation
- Structural analysis of desorbed hemoglobin from nano particle surface
- Functional assay of human hemoglobin and its variants
- Application of native mas spectrometry to calculate equilibrium constant
- Bioconjugation of para-benzoquinone of cigarette smoke with human hemoglobin
- Bioconjugation of human hemoglobin with quinone as airborne enviroment pollutants
- Ion mobility mass spectrometry
- Introduction to hydrogen-deuterium-exchange mass spectrometry
- Rate law of hydrogen-deuterium-exchange kinetics
- Molecular insights in inhibition of HbS polymerisation upon oxygenation monitored by HDX-MS platform-part-I
- Molecular insights in inhibition of HbS polymerisation upon oxygenation monitored by HDX-MS platform-part-II
- Molecular insights in inhibition of HbS polymerisation upon oxygenation -part-III- and upon glutathionylation-part-I-monitored by HDX-MS platform
- Molecular insights in inhibition of HbS polymerisation upon glutathionylation-part-II
- N-acetyl cysteine as an antisickling agent in sickle cell disease
- Effect of quinones on morphology of human RBC
- Effect-of-NAC-to neturalise-quinone-and-in-vivo-HDX with human RBCs as a model system
- Application of HDX-MS in structural analysis of human hemoglobin in vivo
- Application of HDX-MS in structural analysis of hemoglobin in vivo-and-desorbed-hemoglobin from nano particle-surface
- Application-of-HDX-MS-and -CD-spectroscopy-to-monitor-structural changes-of human hemoglobin from nano particle surface
- Structure-function correlation of Hb Beckman, a genetic variant of human hemoglobin, using HDX-MS
- Structure-function correlation of glutathionyl hemoglobin, a posttranslational variant of human hemoglobin, using HDX-MS
- Imaging mass spectromery of biological tissue samples using MALDI mass spectrometry platform
- Kidney tissue imaging in a nano particle exposed rat model
- Imaging of kidney tissues of NP exposed rats and imaging of human prostate tissue
- Imaging of human prostate cancer tissue using MALDI-MS
- Imaging of human prostate cancer tissue using MALDI-MS and analysis of tissue proteomics data
- Desorption Electrospary Ionisation based Mass Spectrometric Imaging of Prostate Cancer Tissue
- Fast photochemical oxidation of protein combined to mass spectrometry for prtein footprinting both in vitro and in vivo
- Biosimilar-monitored through mass spectrometric techniques