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Course12710539166 lectures

Comprehensive Molecular Diagnostics and Advanced Gene Expression Analysis

Prof. Aritri Bir Prof. Arindam Ghosh · IIT Kharagpur

Start with lecture 1 At NPTEL

Lectures

week-01

  1. Lecture 01 : Foundation of Molecular Biology Video
  2. Lecture 02 : DNA Replication & Repair Mechanism Video
  3. Lecture 03 : Transcription ( RNA Synthesis ) Video
  4. Lecture 04: Protein Synthesis (Translation) Video
  5. Lecture 05 : Nucleic Acid Isolation and Detection Methods Video

week-02

  1. Lecture 06 : PCR (Polymerase Chain Reaction) Fundamentals Video
  2. Lecture 07 : Realtime PCR (Part 1) Video
  3. Lecture 08 : Realtime PCR (Part 2) Video
  4. Lecture 09 : Variations of the PCR Video
  5. Lecture 10 : Variations of the PCR (Part 2) Video

week-03

  1. Lecture 11 : DNA Cloning and Recombinant DNA technology -I Video
  2. Lecture 12 : DNA Cloning and Recombinant DNA technology -II Video
  3. Lecture 13 : DNA Cloning and Recombinant DNA technology - III Video
  4. Lecture 14 : Genome Editing Technologies I Video
  5. Lecture 15 : Genome Editing Technologies II Video

week-04

  1. Lecture 16 : DNA Microarray Video
  2. Lecture 17 : FISH ( Fluorescence in situ Hybridization) Video
  3. Lecture 18 : Methods to study DNA-protein interaction I Video
  4. Lecture 19 : Methods to study DNA-protein interaction II Video
  5. Lecture 20 : Epigenetics & DNA methylation analysis Video

week-05

  1. Lecture 21 : DNA Sequencing (Part1) - Maxam Gilbert Sequencing Video
  2. Lecture 22 : DNA Sequencing (Part 2) - Sanger Sequencing Video
  3. Lecture 23 : Next Generation Sequencing Video
  4. Lecture 24 : Next Generation Sequencing (Part 2) Video
  5. Lecture 25 : Next Generation Sequencing (Part 3) Video

week-06

  1. Lecture 26 : Overview, Importance, Methods in Proteomics Video
  2. Lecture 27 : Proteomic Techniques- High performance liquid chromatography (HPLC) Video
  3. Lecture 28 : Proteomic Techniques - Mass Spectrometry Video
  4. Lecture 29 : Proteomic Techniques – Differential in-gel electrophoresis Video
  5. Lecture 30 : Proteomic Techniques – Protein Microarray Video

week-07

  1. Lecture 31 : Label-based Protein Quantification Technologies (Part 1) Video
  2. Lecture 32 : Label-based Protein Quantification Technologies (Part 2) Video
  3. Lecture 33 : Label free methods of protein quantification Video
  4. Lecture 34 : Next Generation Proteomics Video
  5. Lecture 35 : Proteomic Data Analysis and Bioinformatic Tools Video

week-08

  1. Lecture 36 : Syndromic Panels and Multiplex Assay : Molecular identification of Microorganism Video
  2. Lecture 37 : Molecular Diagnostics in Antimicrobial Resistance Testing Video
  3. Lecture 38 : RNA Sequencing: Role in Infectious diseases I Video
  4. Lecture 39 : RNA Sequencing: Role in Infectious diseases II Video
  5. Lecture 40 : Point of Care (POC) diagnostics in Infectious diseases Video

week-09

  1. Lecture 41 : Molecular genetics in tumorogenesis Video
  2. Lecture 42 : Liquid biopsies in cancer detection Video
  3. Lecture 43 : Digital PCR in Cancer detection Video
  4. Lecture 44 : Mutation Detection Methods Video
  5. Lecture 45 : Isothermal Nucleic Acid Amplification Video

week-10

  1. Lecture 46 : Genetic Testing and Inherited Disorders (Part 1) Video
  2. Lecture 47 : Genetic Testing and Inherited Disorders (Part 2) Video
  3. Lecture 48 : Prenatal diagnostics and NIPT Video
  4. Lecture 49 : Reproductive genetics and genetic counselling Video
  5. Lecture 50 : Genetic counselling and patient education Video

week-11

  1. Lecture 51 : Serial Analysis of Gene Expression Video
  2. Lecture 52 : Metabolomics in Molecular Diagnostics Video
  3. Lecture 53 : Immunoassay & Luminex Multiplex Assay Video
  4. Lecture 54: Molecular diagnostics in Metabolic,Cardiovascular and Gastrointestinal disorders Video
  5. Lecture 55 : Molecular diagnostics in Endocrine, , Neurodegenerative, and Transplantation disorders. Video

week-12

  1. Lecture 56 : Pharmacogenomics and Personalized Medicine Video
  2. Lecture 57 : Quality control (QC) in molecular diagnostics Video
  3. Lecture 58 : Ethical concerns in molecular diagnostics Video
  4. Lecture 59 : Artificial Intelligence and Machine learning in Genomics Video
  5. Lecture 60 : Integration of Multiomics Data in Molecular Diagnostics Video

Live Session

  1. Live Session 27-02-2024 Video
  2. Live Session 26-03-2024 Video
  3. Live Session 16-04-2024 Video
  4. Live Session 21-02-2025 Video
  5. Live Session 25-03-2025 Video
  6. Live Session 16-04-2025 Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Kharagpur
Department
Multidisciplinary
Teacher
Prof. Aritri Bir Prof. Arindam Ghosh
Level
Undergraduate
Language
English
Subjects
Mathematics, Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
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