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Course10210702841 lectures

Analytical Technologies in Biotechnology

Dr. Ashwani K. Sharma · IIT Roorkee

Start with lecture 1 At NPTEL

Lectures

Microscopy

  1. Basic concepts in microscopy 1 Video
  2. Basic concepts in microscopy 2 Video
  3. Dark-field and phase contrast microscopy Video
  4. Differential interference contrast and polarization Video
  5. Fluorescence and confocal microscopy Video
  6. Transmission electron microscopy Video
  7. Transmission electron microscopy cont. and scanning electron microscopy Video

Radioisotopes Techniques

  1. Basic concepts 1 Video
  2. Basic concepts 2 Video
  3. GM counting and Scintillation counting Video
  4. Scintillation counting continued Video
  5. Autoradiography and RIA Video
  6. Safety aspects and applications Video

Chromatographic methods

  1. Introduction and Basic concepts in chromatography 1 Video
  2. Basic concepts in chromatography 2 Video
  3. Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography (HPLC) Video
  4. Ion-exchange chromatography Video
  5. Gel-filtration chromatography Video
  6. Affinity chromatography Video
  7. Gas-liquid chromatography Video

Electrophoresis

  1. Basic concepts in electrophoresis Video
  2. Horizontal and vertical gel electrophoresis Video
  3. Native gel electrophoresis and SDS-PAGE Video
  4. Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods Video
  5. Electrophoresis of nucleic acids Video
  6. Immunoelectrophoresis and capillary electrophoresis Video

Centrifugation Techniques

  1. Introduction and basic concepts 1 Video
  2. Basic concepts-2 Video
  3. Types of centrifuges and analytical ultracentrifugation method Video
  4. Separation methods in preparative ultracentrifuges Video
  5. Types of rotors Video
  6. Types of rotors cont. and care of rotors Video

Spectroscopic techniques

  1. Introduction and basic concepts Video
  2. UV-Visible spectroscopy Video
  3. Infrared and fluorescence spectroscopy Video
  4. Circular dichroism (CD) spectroscopy Video
  5. Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography Video
  6. Atomic spectroscopy and mass spectrometry Video

Polymerase chain reaction

  1. Polymerase chain reaction(PCR) Video
  2. DNA sequencing methods Video
  3. Enzyme linked immunosorbent assay (ELISA) Video

Syllabus and materials

At NPTEL, as the course publishes them.

Credits and licence

Institution
IIT Roorkee
Department
Biotechnology
Teacher
Dr. Ashwani K. Sharma
Level
Undergraduate
Language
English
Subjects
Biology
Source
NPTEL
Licence
CC BY-NC-SA, as NPTEL states it. Non-commercial use only. NPTEL's terms
Attribution
NPTEL states: "Distributed under Creative Commons Attribution-ShareAlike / CC BY - NC - SA", beside a BY-NC-SA badge.
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