Learn · Analytical Technologies in Biotechnology

Lecture 16 of 41Chromatographic methods

Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography (HPLC)

Dr. Ashwani K. Sharma · IIT Roorkee

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Analytical Technologies in Biotechnology, IIT Roorkee (at NPTEL)
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CC BY-NC-SA, as NPTEL states it. Non-commercial use only.
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All the lectures

  1. Basic concepts in microscopy 1 Video
  2. Basic concepts in microscopy 2 Video
  3. Dark-field and phase contrast microscopy Video
  4. Differential interference contrast and polarization Video
  5. Fluorescence and confocal microscopy Video
  6. Transmission electron microscopy Video
  7. Transmission electron microscopy cont. and scanning electron microscopy Video
  8. Basic concepts 1 Video
  9. Basic concepts 2 Video
  10. GM counting and Scintillation counting Video
  11. Scintillation counting continued Video
  12. Autoradiography and RIA Video
  13. Safety aspects and applications Video
  14. Introduction and Basic concepts in chromatography 1 Video
  15. Basic concepts in chromatography 2 Video
  16. Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography (HPLC) Video
  17. Ion-exchange chromatography Video
  18. Gel-filtration chromatography Video
  19. Affinity chromatography Video
  20. Gas-liquid chromatography Video
  21. Basic concepts in electrophoresis Video
  22. Horizontal and vertical gel electrophoresis Video
  23. Native gel electrophoresis and SDS-PAGE Video
  24. Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods Video
  25. Electrophoresis of nucleic acids Video
  26. Immunoelectrophoresis and capillary electrophoresis Video
  27. Introduction and basic concepts 1 Video
  28. Basic concepts-2 Video
  29. Types of centrifuges and analytical ultracentrifugation method Video
  30. Separation methods in preparative ultracentrifuges Video
  31. Types of rotors Video
  32. Types of rotors cont. and care of rotors Video
  33. Introduction and basic concepts Video
  34. UV-Visible spectroscopy Video
  35. Infrared and fluorescence spectroscopy Video
  36. Circular dichroism (CD) spectroscopy Video
  37. Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography Video
  38. Atomic spectroscopy and mass spectrometry Video
  39. Polymerase chain reaction(PCR) Video
  40. DNA sequencing methods Video
  41. Enzyme linked immunosorbent assay (ELISA) Video